Abstract

The analysis of matrix attachment regions (MARs) associated with the lymphocyte receptors has provided a link between nuclear matrix attachment and a number of activities that regulate gene expression. We identified a nuclear matrix- associated, B cell-restricted regulator of IgH transcription, Bright. Bright binds as a tetramer to MARs necessary for matrix attachment of the IgH intronic enhancer (Emu) and the VHS107 promoter. These interactions lead to DNA bending, enhanced chromatin accessibility, and transcriptional activation. As with the nuclear matrix, transcription take place in defined subnuclear topological domains. We have identified and characterized several heteromeric interactions of Bright that provide a link between one such domain, the PML nuclear body (NB), matrix attachment and transcription of the IgH locus. We propose to extend our work by (1) further characterizing the structure, function, and expression of Bright; (2) determining the functional consequences for interactions of Bright with PML NB proteins that lead to alteration of its activity and localization; and (3) investigating the mechanism by which Bright contributes to chromatin remodeling.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA031534-21
Application #
6710126
Study Section
Allergy and Immunology Study Section (ALY)
Program Officer
Mccarthy, Susan A
Project Start
1982-03-01
Project End
2007-03-31
Budget Start
2004-04-01
Budget End
2005-03-31
Support Year
21
Fiscal Year
2004
Total Cost
$267,000
Indirect Cost

  Institution

Name
University of Texas Austin
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
170230239
City
Austin
State
TX
Country
United States
Zip Code
78712

  Publications

Warren, Junco S; Tracy, Christopher M; Miller, Mickey R et al. (2018) Histone methyltransferase Smyd1 regulates mitochondrial energetics in the heart. Proc Natl Acad Sci U S A 115:E7871-E7880
Nie, Hui; Rathbun, Gary; Tucker, Haley (2017) Smyd1C Mediates CD8 T Cell Death via Regulation of Bcl2-Mediated Restriction of outer Mitochondrial Membrane Integrity. J Cell Signal 2:
Rhee, Catherine; Edwards, Melissa; Dang, Christine et al. (2017) ARID3A is required for mammalian placenta development. Dev Biol 422:83-91
Rhee, Catherine; Kim, Jonghwan; Tucker, Haley O (2017) Transcriptional Regulation of the First Cell Fate Decision. J Dev Biol Regen Med 1:
Kim, Dongkyoon; Schmidt, Christian; Brown, Mark A et al. (2017) Competitive Promoter-Associated Matrix Attachment Region Binding of the Arid3a and Cux1 Transcription Factors. Diseases 5:
Li, Hanjun; Liu, Pei; Xu, Shuqin et al. (2017) FOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging. J Clin Invest 127:1241-1253
Li, Shanru; Morley, Michael; Lu, MinMin et al. (2016) Foxp transcription factors suppress a non-pulmonary gene expression program to permit proper lung development. Dev Biol 416:338-46
Woodworth, Mollie B; Greig, Luciano C; Liu, Kevin X et al. (2016) Ctip1 Regulates the Balance between Specification of Distinct Projection Neuron Subtypes in Deep Cortical Layers. Cell Rep 15:999-1012
Huang, Ching-Jung; Das, Utsab; Xie, Weijun et al. (2016) Altered stoichiometry and nuclear delocalization of NonO and PSF promote cellular senescence. Aging (Albany NY) 8:3356-3374
Kim, Peter Geon; Canver, Matthew C; Rhee, Catherine et al. (2016) Interferon-? signaling promotes embryonic HSC maturation. Blood 128:204-16

Showing the most recent 10 out of 54 publications