Abstract

This is a basic and clinical study to elucidate the mechanisms of action of hematoporophyrin derivative plus light in the treatment and diagnosis of cancer. Three basic cell areas of investigation are proposed; 1. Further clarifying the cellular sites of binding; 2. Identifying the sites of phototoxicity (just because HpD binds to a particular site does not mean that that is the site of phototoxicity); 3. Identifying which HpD subcomponents are phototoxic and which are fluorescent (some may be both). In vitro studies will employ malignant and non-malignant cell lines with particular emphasis on toxicity and sites of binding structural alterations. Techniques used will be fluorescence detection, electron microscopy, and tissue culture. Animal studies will involve the use of different tumor systems with the main objectives being pathological and fluorescence analysis of HpD and light effects. Diagnostic studies will be done on human patients employing fluorescence detection in vivo plus serial biopsies followed by fluorescence analysis. Human treatment will involve treating patients with head and neck, gynecological, and bladder cancer.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
2R01CA032248-04A1
Application #
3170224
Study Section
Diagnostic Radiology Study Section (RNM)
Project Start
1982-05-01
Project End
1988-12-31
Budget Start
1986-02-01
Budget End
1986-12-31
Support Year
4
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of California Irvine
Department
Type
Schools of Medicine
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Degen, Andrea F; Gabrecht, Tanja; Mosimann, Laurent et al. (2004) Photodynamic endometrial ablation for the treatment of dysfunctional uterine bleeding: a preliminary report. Lasers Surg Med 34:1-4
Kimel, Sol; Svaasand, Lars O; Hammer-Wilson, Marie J et al. (2003) Influence of wavelength on response to laser photothermolysis of blood vessels: implications for port wine stain laser therapy. Lasers Surg Med 33:288-95
Keefe, Kristin A; Tadir, Yona; Tromberg, Bruce et al. (2002) Photodynamic therapy of high-grade cervical intraepithelial neoplasia with 5-aminolevulinic acid. Lasers Surg Med 31:289-93
Hammer-Wilson, Marie J; Cao, Danielle; Kimel, Sol et al. (2002) Photodynamic parameters in the chick chorioallantoic membrane (CAM) bioassay for photosensitizers administered intraperitoneally (IP) into the chick embryo. Photochem Photobiol Sci 1:721-8
Chan, John K; Monk, Bradley J; Cuccia, David et al. (2002) Laparoscopic photodynamic diagnosis of ovarian cancer using 5-aminolevulinic acid in a rat model. Gynecol Oncol 87:64-70
Kimel, Sol; Svaasand, Lars O; Cao, Danielle et al. (2002) Vascular response to laser photothermolysis as a function of pulse duration, vessel type, and diameter: implications for port wine stain laser therapy. Lasers Surg Med 30:160-9
Keefe, K A; Chahine, E B; DiSaia, P J et al. (2001) Fluorescence detection of cervical intraepithelial neoplasia for photodynamic therapy with the topical agents 5-aminolevulinic acid and benzoporphyrin-derivative monoacid ring. Am J Obstet Gynecol 184:1164-9
Pham, T H; Hornung, R; Berns, M W et al. (2001) Monitoring tumor response during photodynamic therapy using near-infrared photon-migration spectroscopy. Photochem Photobiol 73:669-77
Liang, H; Do, T; Kasravi, S et al. (2000) Chromosomes are target sites for photodynamic therapy as demonstrated by subcellular laser microirradiation. J Photochem Photobiol B 54:175-84
Tadir, Y; Hornung, R; Pham, T H et al. (1999) Intrauterine light probe for photodynamic ablation therapy. Obstet Gynecol 93:299-303

Showing the most recent 10 out of 65 publications