This project seeks to improve the current methods of labeling leukocytes and platelets with gamma-emitting agents for the delineation of focal inflammatory lesions, endothelial injury and thrombi by scintillation camera imaging. New radiopharmaceuticals will be developed, including analogues of 8-hydroxyquinoline, disulphide compounds and bis-thiosemicarbazones. These lipophilic chelating agents will be labeled with 99mTc, 111In, 197Hg, 203Pb or 97Ru. A continuous flow elutriation system with a special centrifuge rotor will be used to harvest leukocytes and centrifugation with Metrizamide or Stractan density gradients will be used to harvest platelets. Viability of the isolated labeled cells will be assessed by reinjection into experimental animals and measurement of cell recovery and survival in the blood stream. Their efficacy in demonstrating focal inflammatory lesions and vascular damage will be assessed in animals by gamma camera imaging and by direct tissue radioassay.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA032853-13
Application #
3170706
Study Section
Diagnostic Radiology Study Section (RNM)
Project Start
1980-01-01
Project End
1990-03-31
Budget Start
1989-01-01
Budget End
1990-03-31
Support Year
13
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Upstate Medical University
Department
Type
Schools of Medicine
DUNS #
058889106
City
Syracuse
State
NY
Country
United States
Zip Code
13210
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