Abstract

This project seeks to improve the current methods of labeling leukocytes and platelets with gamma-emitting agents for the delineation of focal inflammatory lesions, endothelial injury and thrombi by scintillation camera imaging. New radiopharmaceuticals will be developed, including analogues of 8-hydroxyquinoline, disulphide compounds and bis-thiosemicarbazones. These lipophilic chelating agents will be labeled with 99mTc, 111In, 197Hg, 203Pb or 97Ru. A continuous flow elutriation system with a special centrifuge rotor will be used to harvest leukocytes and centrifugation with Metrizamide or Stractan density gradients will be used to harvest platelets. Viability of the isolated labeled cells will be assessed by reinjection into experimental animals and measurement of cell recovery and survival in the blood stream. Their efficacy in demonstrating focal inflammatory lesions and vascular damage will be assessed in animals by gamma camera imaging and by direct tissue radioassay.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA032853-15
Application #
3170707
Study Section
Diagnostic Radiology Study Section (RNM)
Project Start
1980-01-01
Project End
1995-02-28
Budget Start
1991-03-01
Budget End
1992-02-29
Support Year
15
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
George Washington University
Department
Type
Schools of Medicine
DUNS #
City
Washington
State
DC
Country
United States
Zip Code
20052

  Publications

Albright, J W; Mease, R C; Lambert, C et al. (1999) Trypanosoma musculi: tracking parasites and circulating lymphoid cells in host mice. Exp Parasitol 91:185-95
Albright, J W; Mease, R C; Lambert, C et al. (1998) Effects of aging on the dynamics of lymphocyte organ distribution in mice: use of a radioiodinated cell membrane probe. Mech Ageing Dev 101:197-211
Lambert, C; Mease, R C; Avren, L et al. (1996) Radioiodinated (aminostyryl)pyridinium (ASP) dyes: new cell membrane probes for labeling mixed leukocytes and lymphocytes for diagnostic imaging. Nucl Med Biol 23:417-27
McAfee, J G; Gagne, G; Subramanian, G et al. (1991) The localization of indium-111-leukocytes, gallium-67-polyclonal IgG and other radioactive agents in acute focal inflammatory lesions. J Nucl Med 32:2126-31
McAfee, J G (1990) What is the best method for imaging focal infections? J Nucl Med 31:413-6
McAfee, J G; Kopecky, R T; Frymoyer, P A (1990) Nuclear medicine comes of age: its present and future roles in diagnosis. Radiology 174:609-20
Ames, I H; Gagne, G M; Garcia, A M et al. (1989) Preferential homing of tumor-infiltrating lymphocytes in tumor-bearing mice. Cancer Immunol Immunother 29:93-100
Garcia, A M; Gagne, G M; Ames, I H et al. (1988) Migratory patterns of different indium-111 labeled leukocyte populations (chiefly lymphocytes) from control and thymectomized rats. J Nucl Med 29:83-90
McAfee, J G (1988) Future directions in radiolabelling leucocytes--their substituents and their microenvironment. Nucl Med Commun 9:775-81
McAfee, J G (1988) Non-inflammatory applications in vivo of radiolabelled leucocytes: a review. Nucl Med Commun 9:733-44

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