The mechanism of action of the antineoplastic agent tricyclic nucleoside [TCN; NSC-154020; 3-amino-1, 5 dihydro-5-methyl-1-(B-D-ribofuranosyl)-1, 4, 5, 6, 8 pentaazaace-naphthylene] will be further studied, using chinese hamster ovary cells in culture and a rabbit reticulocyte lysate protein synthesizing system. The length of exposure required to kill cells will be determined; the mechanism of inhibition of purine biosynthesis will be examined; the point of inhibition of protein synthesis will be determined.
| Moore, E C; Hurlbert, R B; Boss, G R et al. (1989) Inhibition of two enzymes in de novo purine nucleotide synthesis by triciribine phosphate (TCN-P). Biochem Pharmacol 38:4045-51 |
| Moore, E C; Hurlbert, R B; Massia, S P (1989) Inhibition of CCRF-CEM human leukemic lymphoblasts by triciribine (tricyclic nucleoside, TCN, NSC-154020). Accumulation of drug in cells and comparison of effects on viability, protein synthesis and purine synthesis. Biochem Pharmacol 38:4037-44 |
