Abstract

The major objective of this grant application is to develop methods to detect AIDS in blood samples for transfusion, the potential to transmit AIDS, so that such blood specimens can be eliminated from the blood donor pool. The study population will consist of 350 male homosexuals of whom 20% are symptom free, 60% have the AIDS-Related Symptom Complex (ARC), and 20% have AIDS with Kaposi's sarcoma, Opportunistic Infection, or both. In addition, 500 (or more if necessary) blood bank donor specimens, 100 primary breast cancer patients, and 100 acute leukemia patients will be studied. The latter patient group will be evaluated at diagnosis and one year later when they usually have received multiple blood transfusions and immunosuppressive chemotherapy. Also, 100 female hospital employees with no AIDS contacts will be additional controls. The premise of the study will be that one single test may either over- or underestimate the potential to transmit AIDS. Therefore, a battery of nine tests will be applied to 15 ml of blood from each donor and we will select two or three parametera which identify 100% of ARC patients with lymphadenopathy, 100% of patients who move from symptom free to ARC or from ARC to AIDS, and 100% of AIDS patients (with the precaution that some of the potentially positive parameters may become negative in patients with advanced disease). The parameters to be studied will include CMV antibody titer (indirect immunofluorescence), antithymic antibody titer (indirect immunofluorescence), immune complexes (Clq biding assay, modified for ELISA), serum alpha-interferon titers (including acid lability by cytopathic effect modification), serum beta-2 microglobulin level (radioimmunoassay), blood levels of cella marking for the T3, T4, and T8 antigens, with calculation of the 4:8 ratio and lymphocytes (flow cytometry), 5'-nucleotidase levels in terms of enzyme activity /10?6? /hr (for conversion of C?14?-AMP substrate). Data will be analyzed by chi-squared and ANOVA tests, and multivariant logistic regression analysis. Evaluation of 500 normal blood bank specimens will allow an estimate of cost effectiveness procedures. Evaluation of transfused leukemia patients will give estimate of clinical risk. (SR)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA034674-03
Application #
3172433
Study Section
Experimental Immunology Study Section (EI)
Project Start
1983-02-07
Project End
1987-01-31
Budget Start
1985-02-01
Budget End
1987-01-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Texas MD Anderson Cancer Center
Department
Type
Hospitals
DUNS #
001910777
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Murray, J L; Bywaters, D W; Reuben, J M et al. (1987) Decreased 5'-nucleotidase activity in suppressor (OKT8) T lymphocytes from homosexuals with AIDS-related complex: nonassociation with enhanced deoxynucleoside toxicity. Clin Immunol Immunopathol 42:10-7
Lepe-Zuniga, J L; Mansell, P W; Hersh, E M (1987) Idiopathic production of interleukin-1 in acquired immune deficiency syndrome. J Clin Microbiol 25:1695-700
Patt, Y Z; Mavligit, G M; Reuben, J et al. (1986) Modulation in vitro of immune parameters in homosexual males with the preclinical complex of symptoms related to acquired immune deficiency syndrome by azimexon. J Biol Response Mod 5:263-9
Sumaya, C V; Boswell, R N; Ench, Y et al. (1986) Enhanced serological and virological findings of Epstein-Barr virus in patients with AIDS and AIDS-related complex. J Infect Dis 154:864-70
Patt, Y Z; Mansell, P W; Reuben, J M et al. (1986) Effect of azimexon therapy on host defense parameters and disease-associated symptoms in the acquired immune deficiency syndrome (AIDS) and AIDS-related complex (ARC). AIDS Res 2:191-209
Reuben, J M; LaRocco, J M; Gschwind, C R et al. (1986) Relationship of exposure to human T lymphotropic virus and T helper cells in homosexual men. AIDS Res 2:117-26
Kalter, S P; Riggs, S A; Cabanillas, F et al. (1985) Aggressive non-Hodgkin's lymphomas in immunocompromised homosexual males. Blood 66:655-9
Reuben, J M; Hersh, E M; Murray, J L et al. (1985) IL-2 production and response in vitro by the leukocytes of patients with acquired immune deficiency syndrome. Lymphokine Res 4:103-16
Murray, J L; Loftin, K C; Munn, C G et al. (1985) Elevated adenosine deaminase and purine nucleoside phosphorylase activity in peripheral blood null lymphocytes from patients with acquired immune deficiency syndrome. Blood 65:1318-24
Rios, A; Mansell, P W; Newell, G R et al. (1985) Treatment of acquired immunodeficiency syndrome--related Kaposi's sarcoma with lymphoblastoid interferon. J Clin Oncol 3:506-12

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