Abstract

The goal of this research is the characterization of human myeloid leukemia cells using murine and human monoclonal antibody techniques to provide reagents that will be useful in the diagnosis and treatment of human myeloid leukemia. Monoclonal antibodies will be produced against cell surface antigens on normal and malignant myeloid cells with emphasis on myeloid precursor cells and their differentiation products. This technology also includes recently developed procedures to expand normal antibody-producing B-lymphocyte cultures in large quantities by demethylation of cytosin in certain nucleotide sequences. The antigenic determinants for such antibodies will be biochemically characterized. Also included will be studies on the modulation potential of such antigens. (2)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA035227-03
Application #
3172843
Study Section
Experimental Immunology Study Section (EI)
Project Start
1983-09-15
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Copenhagen
Department
Type
DUNS #
City
Copenhagen
State
Country
Denmark
Zip Code

  Publications

Olsson, L; Behnke, O (1988) Emergence of a retrovirus in a cloned cell line established from a lesion of Hodgkin's disease. Hematol Oncol 6:213-22
Due, C; Eriksson, H; Sihm, A et al. (1987) Association between expression of a tumor-associated carbohydrate epitope and the proliferative and tumorigenic activity of a human squamous lung cancer cell line and epitope-positive and -negative sublines. Cancer Res 47:6697-704
Andreasen, R B; Olsson, L (1987) Identification and characterization of an antigen specific for normal erythroid precursor cells and its application in diagnosis of erythroleukemia. Leuk Res 11:1045-53
Stagsted, J; Olsson, L (1987) Antibody-induced modulation of H-2Kb antigens on mouse tumor cells in vitro. Cancer Detect Prev Suppl 1:65-71
Brams, P; Pettijohn, D E; Brown, M et al. (1987) In vitro B-lymphocyte antigen priming against both non-immunogenic and immunogenic molecules requiring low amounts of antigen and applicable in hybridoma technology. J Immunol Methods 98:11-22
Due, C; Simonsen, M; Olsson, L (1986) The major histocompatibility complex class I heavy chain as a structural subunit of the human cell membrane insulin receptor: implications for the range of biological functions of histocompatibility antigens. Proc Natl Acad Sci U S A 83:6007-11
Rahbek Sorensen, H; Olsson, L (1986) On the advent and necessity of molecular biology in the clinical management of lung cancer. Thorac Cardiovasc Surg 34:345-50
Honsik, C J; Diamant, M; Olsson, L (1986) Generation of stable cellular phenotypes in a human malignant cell line conditioned by alterations in the cellular microenvironment. Cancer Res 46:940-9
Klokker, M; Jessen, H; Olsson, L et al. (1986) Morphological features of established cultures of human squamous lung carcinoma cells and the cellular distribution of tumor-specific glycoproteins. Acta Pathol Microbiol Immunol Scand A 94:381-90
Andreasen, R B; Biberfeld, P; Ost, A et al. (1986) Specificity and diagnostic implications of the reactivity pattern of a panel of monoclonal antibodies against myeloid leukemia cells. Scand J Haematol 37:323-32

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