Abstract

The specific aims of this proposal are to: (1) Characterize mos-associated proteins by multiple approaches including co-fractionation with epitope-tagged mos, coprecipitation with mos, binding assays with mos immobilized on nitrocellulose paper. (2) Identify mos-interacting proteins by using improved yeast two-hybrid system. (3) Identify p35CdK, which associates with v-mos in MSV transformed NIH3T3 cells, and determine the physiological significance of this interaction. (4) Analyze the mechanism of activation of MAP kinase (MEK 1 and 2) in v-mos transformed cells. (5) Determine the relationship of v-mos to a signal transduction pathway involving ras and raf-1 by identification of additional v-mos substrates. (6) Study the effect of v-mos kinase on the activity of various CdKS.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
2R01CA045125-09
Application #
2091733
Study Section
Virology Study Section (VR)
Project Start
1986-09-30
Project End
1998-06-30
Budget Start
1995-07-20
Budget End
1996-06-30
Support Year
9
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
University of Texas MD Anderson Cancer Center
Department
Pathology
Type
Other Domestic Higher Education
DUNS #
001910777
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Pham, C D; Vuyyuru, V B; Yang, Y et al. (1999) Evidence for an important role of serine 16 and its phosphorylation in the stabilization of c-Mos. Oncogene 18:4287-94
Liu, H; Vuyyuru, V B; Pham, C D et al. (1999) Evidence of an interaction between Mos and Hsp70: a role of the Mos residue serine 3 in mediating Hsp70 association. Oncogene 18:3461-70
Yang, Y; Pham, C D; Arlinghaus, R B et al. (1998) Elevated level of cyclin D1 in mos-transformed cells. Int J Oncol 12:1199-202
Yang, Y; Pham, C D; Vuyyuru, V B et al. (1998) Evidence of a functional interaction between serine 3 and serine 25 Mos phosphorylation sites. A dominant inhibitory role of serine 25 phosphorylation on Mos protein kinase. J Biol Chem 273:15946-53
Singh, B; Arlinghaus, R B (1997) Mos and the cell cycle. Prog Cell Cycle Res 3:251-9
Gao, C; Arlinghaus, R B; Singh, B (1996) Further characterization of the c-mos transcript and its cell cycle specific expression in NIH3T3 cells. Oncogene 12:1571-6
Yang, Y; Herrmann, C H; Arlinghaus, R B et al. (1996) Inhibition of v-Mos kinase activity by protein kinase A. Mol Cell Biol 16:800-9
Pham, C D; Arlinghaus, R B; Zheng, C F et al. (1995) Characterization of MEK1 phosphorylation by the v-Mos protein. Oncogene 10:1683-8
Bai, W; Arlinghaus, R B; Singh, B (1993) Association of v-Mos with soluble vimentin in vitro and in transformed cells. Oncogene 8:2207-12
Bai, W; Singh, B; Yang, Y et al. (1992) Evidence for interaction between v-Mos and a p34cdc2 isoform, p35cdk. Oncogene 7:1757-63

Showing the most recent 10 out of 26 publications