Abstract

Dr. Beemon proposes a series of studies to examine the regulation of processing of ALV/Rouse sarcoma virus (RSV) RNA.
In Aim 1, a recently discovered CTE will be characterized. The main goal will be to identify and clone a cDNA encoding a cellular protein that binds to the CTE and is responsible for its activity. Xenopus oocyte injections will be used to test for function.
In Aim 2, a negative regulator of splicing in the gag gene of RSV will be analyzed to determine what cellular splicing factors bind to it and what roles these might play in inhibiting splicing.
In Aim 3 a set of studies will be performed to examine the role that deletion of a 42 bp sequence in the NRS plays in conferring pathogenicity on a variant of ALV that induces high incidence, rapid onset B cell lymphoma by insertional activation of c-Myb. Experiments will be performed to test whether deletion of this part of the NRS affects readthrough of viral transcripts and splicing to an acceptor in the c-Myb gene.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA048746-12
Application #
6150086
Study Section
Experimental Virology Study Section (EVR)
Program Officer
Cole, John S
Project Start
1988-12-01
Project End
2003-01-31
Budget Start
2000-02-08
Budget End
2001-01-31
Support Year
12
Fiscal Year
2000
Total Cost
$356,812
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Balagopal, Vidya; Beemon, Karen L (2017) Rous Sarcoma Virus RNA Stability Element Inhibits Deadenylation of mRNAs with Long 3'UTRs. Viruses 9:
Ge, Zhiyun; Quek, Bao Lin; Beemon, Karen L et al. (2016) Polypyrimidine tract binding protein 1 protects mRNAs from recognition by the nonsense-mediated mRNA decay pathway. Elife 5:
Justice 4th, James F; Morgan, Robin W; Beemon, Karen L (2015) Common Viral Integration Sites Identified in Avian Leukosis Virus-Induced B-Cell Lymphomas. MBio 6:e01863-15
Malhotra, Sanandan; Justice 4th, James; Lee, Nathan et al. (2015) Complete genome sequence of an american avian leukosis virus subgroup j isolate that causes hemangiomas and myeloid leukosis. Genome Announc 3:
Justice 4th, James; Malhotra, Sanandan; Ruano, Miguel et al. (2015) The MET gene is a common integration target in avian leukosis virus subgroup J-induced chicken hemangiomas. J Virol 89:4712-9
Quek, Bao Lin; Beemon, Karen (2014) Retroviral strategy to stabilize viral RNA. Curr Opin Microbiol 18:78-82
Justice 4th, James; Beemon, Karen L (2013) Avian retroviral replication. Curr Opin Virol 3:664-9
Leblanc, Jason; Weil, Jason; Beemon, Karen (2013) Posttranscriptional regulation of retroviral gene expression: primary RNA transcripts play three roles as pre-mRNA, mRNA, and genomic RNA. Wiley Interdiscip Rev RNA 4:567-80
Withers, Johanna B; Ashvetiya, Tamara; Beemon, Karen L (2012) Exclusion of exon 2 is a common mRNA splice variant of primate telomerase reverse transcriptases. PLoS One 7:e48016
Bolisetty, Mohan; Blomberg, Jonas; Benachenhou, Farid et al. (2012) Unexpected diversity and expression of avian endogenous retroviruses. MBio 3:e00344-12

Showing the most recent 10 out of 37 publications