Abstract

The long term objective of the proposed research is to develop minimally invasive techniques for the detection of precancerous changes in tissue utilizing laser induced fluorescence (LIF). In this proposal, endoscope-compatible clinical systems will be developed for real time spectroscopic diagnosis of transitional cell carcinoma (TCC) in the urinary bladder, and colorectal dysplasia/carcinoma in chronic mucosal ulcerative colitis (MUC) and other settings. LEF from these selected tissues will be characterized, the spectroscopic features will be correlated with tissue morphology from fluorescence microscopy and microspectrofluorimetry studies, and decision schemes for accurate diagnosis of disease will be established utilizing morphologic/molecular models. The techniques developed in this investigation will be broadly applicable to the characterization of disease in many other tissue systems, and in the long term will provide the capability for real time in vivo diagnosis. This proposal focuses on the development of these methodologies for the direction of biopsy in the detection of precancerous chances in these tissues.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
1R01CA053717-01
Application #
3198381
Study Section
Diagnostic Radiology Study Section (RNM)
Project Start
1990-12-21
Project End
1993-11-30
Budget Start
1990-12-21
Budget End
1991-11-30
Support Year
1
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
Organized Research Units
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Georgakoudi, Irene; Feld, Michael S (2004) The combined use of fluorescence, reflectance, and light-scattering spectroscopy for evaluating dysplasia in Barrett's esophagus. Gastrointest Endosc Clin N Am 14:519-37, ix
Georgakoudi, Irene; Van Dam, Jacques (2003) Characterization of dysplastic tissue morphology and biochemistry in Barrett's esophagus using diffuse reflectance and light scattering spectroscopy. Gastrointest Endosc Clin N Am 13:297-308
Georgakoudi, Irene; Jacobson, Brian C; Muller, Markus G et al. (2002) NAD(P)H and collagen as in vivo quantitative fluorescent biomarkers of epithelial precancerous changes. Cancer Res 62:682-7
Gurjar, R S; Backman, V; Perelman, L T et al. (2001) Imaging human epithelial properties with polarized light-scattering spectroscopy. Nat Med 7:1245-8
Georgakoudi, I; Jacobson, B C; Van Dam, J et al. (2001) Fluorescence, reflectance, and light-scattering spectroscopy for evaluating dysplasia in patients with Barrett's esophagus. Gastroenterology 120:1620-9
Wallace, M B; Perelman, L T; Backman, V et al. (2000) Endoscopic detection of dysplasia in patients with Barrett's esophagus using light-scattering spectroscopy. Gastroenterology 119:677-82
Wang, T D; Crawford, J M; Feld, M S et al. (1999) In vivo identification of colonic dysplasia using fluorescence endoscopic imaging. Gastrointest Endosc 49:447-55
Boustany, N N; Crawford, J M; Manoharan, R et al. (1999) Analysis of nucleotides and aromatic amino acids in normal and neoplastic colon mucosa by ultraviolet resonance raman spectroscopy. Lab Invest 79:1201-14
Zonios, G; Cothren, R; Crawford, J M et al. (1998) Spectral pathology. Ann N Y Acad Sci 838:108-15
Cothren, R M; Sivak Jr, M V; Van Dam, J et al. (1996) Detection of dysplasia at colonoscopy using laser-induced fluorescence: a blinded study. Gastrointest Endosc 44:168-76

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