The objective of this proposal is to develop and improve endoscopic-compatible spectroscopic techniques based on excitation-emission matrix (EEM) fluorescence, reflectance and light scattering for real-time in vivo analysis and diagnosis of dysplasia in the colon, esophagus and urinary bladder. We have developed three models to analyze tissue reflectance and fluorescence. The diffuse reflectance model furnishes information about tissue scatterers (such as mitochondria and the fiber network of connective tissue) and absorbers (such as hemoglobin). The light scattering spectroscopy (LSS) model yields information about nuclear size and crowding in the epithelial layer of tissue. The intrinsic fluorescence model isolates the contributions of fluorophores (such as structural proteins, NADH, porphyrins etc.) from the interference of absorbers and scatterers. Clinical EEM fluorescence/reflectance data will be collected for colonic adenoma, Barrett's esophagus and urothelial carcinoma, and will be analyzed using these models. Information about tissue structure and composition will be extracted and used to formulate new models and diagnostic algorithms. Validation of the algorithms will be performed prospectively with extended clinical data sets. In addition, we will extend the excitation wavelength range of our clinical EEM system down to 308 nm, to access more information about epithelial cellular proteins, update our data acquisition systems, and integrate data analysis software for real-time diagnosis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
2R01CA053717-10
Application #
6129310
Study Section
Diagnostic Imaging Study Section (DMG)
Program Officer
Song, Min-Kyung H
Project Start
1990-12-21
Project End
2003-03-31
Budget Start
2000-04-01
Budget End
2001-03-31
Support Year
10
Fiscal Year
2000
Total Cost
$369,743
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
Organized Research Units
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
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Georgakoudi, Irene; Van Dam, Jacques (2003) Characterization of dysplastic tissue morphology and biochemistry in Barrett's esophagus using diffuse reflectance and light scattering spectroscopy. Gastrointest Endosc Clin N Am 13:297-308
Georgakoudi, Irene; Jacobson, Brian C; Muller, Markus G et al. (2002) NAD(P)H and collagen as in vivo quantitative fluorescent biomarkers of epithelial precancerous changes. Cancer Res 62:682-7
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Cothren, R M; Sivak Jr, M V; Van Dam, J et al. (1996) Detection of dysplasia at colonoscopy using laser-induced fluorescence: a blinded study. Gastrointest Endosc 44:168-76

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