Equine infectious anemia virus (EIAV) is a member of the lentivirus subfamily of retroviruses. EIAV causes a unique disease characterized by repeated bouts of fever and viremia. Virus isolates recovered from sequential febrile episodes from the same animal can be distinguished serologically and biochemically, indicating that viral mutation plays a key role in persistence. Other features of EIAV infection include a short latent period prior to disease onset, the persistence of virus in the presence of competent host immune responses, and the eventual control of disease in many animals. Our long term goals are to understand the nature of EIAV persistence and pathogenesis as well as the dynamic interactions between virus and host that result in an episodic disease. The short latent period prior to disease onset makes EIAV a powerful tool for mutagenesis-based studies of pathogenesis as well as an ideal model in which to determine how viral mutation rates impact on the efficacy of vaccines and antiviral chemotherapies. The proposed studies focus on recovery of molecular clones of virulent EIAV strains and identification of those genetic elements that play key roles in tissue tropism and disease production.
The specific aims of this proposal include: The recovery and characterization of virulent proviral clones of EIAV. Functional analysis of EIAV gene products and cis/acting sequences and their contributions to viral replication and disease. Determination of viral factors that influence cell tropism.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
2R01CA059278-06A1
Application #
2384925
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1993-03-01
Project End
2001-01-31
Budget Start
1997-04-01
Budget End
1998-01-31
Support Year
6
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Texas Arlington
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
064234610
City
Arlington
State
TX
Country
United States
Zip Code
76019
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