The WT1 protein is a sequence-specific DNA-binding transcription factor with an critical role in normal kidney development and the pathogenesis of Wilms' tumor. During the initial funding period the investigators established that WT1 may act as an activator of transcription and that dominant negative forms of WT1 can block activation. The investigators further demonstrated that WT1 can self associate and proposed that non-DNA binding, dominant-negative forms of the WT1 protein shield or sequester wild-type WT1. However, the role of WTl multimerization in its normal function to activate or repress transcription is not clear. The ability of WT1 to activate or repress transcription depends on the nature of the expression vector used in co-transfection experiments. The investigators believe that WT1 is a default activator of transcription and under experimental conditions that deplete WT1 of essential co-factors for activation, WT1 will repress a target gene. Despite much study, the mechanism by which gene regulation is modulated by WT1 remains obscure and the nature of the transcriptional co-factors required for WT1 function is not yet known. In addition it is not known which transcriptional function of WT 1 is critical for its role in controlling differentiation and cellular growth. Tumor associated mutations in WT1 have been isolated in both its minimal activation domain and repression domain. They believe that these mutations interfere with critical protein-protein interactions of WT1 and may serve to genetically identify critical co-factors required for the action of the WT1 protein. During the initial term of this grant they found that WT1 can repress growth and induce epithelial-like differentiation of fibroblasts. This offers us a system in which to study the biological effects of tumor-associated and artificial mutations of WT 1. Such a system may also allow us to determine the biological importance of proteins that closely associate with WT1. Through these aims, by the end of the next period of funding, they will have a much more detailed understanding of the mechanism of action of WT1 in gene regulation, growth suppression and induction of differentiation. They hope to characterize the mechanism(s) by which WT1 represses target genes and identify proteins that interact with WT1 to modulate both its transcriptional and biological effects.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA059998-05
Application #
2633854
Study Section
Pathology B Study Section (PTHB)
Program Officer
Gallahan, Daniel L
Project Start
1994-01-01
Project End
2000-12-31
Budget Start
1998-01-01
Budget End
1998-12-31
Support Year
5
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Mount Sinai School of Medicine
Department
Biochemistry
Type
Schools of Medicine
DUNS #
114400633
City
New York
State
NY
Country
United States
Zip Code
10029
Nabet, Behnam; Ó Broin, Pilib; Reyes, Jaime M et al. (2015) Deregulation of the Ras-Erk Signaling Axis Modulates the Enhancer Landscape. Cell Rep 12:1300-13
Kuracha, Murali R; Siefker, Ed; Licht, Jonathan D et al. (2013) Spry1 and Spry2 are necessary for eyelid closure. Dev Biol 383:227-38
Hwangpo, Tracy Anh; Jordan, J Dedrick; Premsrirut, Prem K et al. (2012) G Protein-regulated inducer of neurite outgrowth (GRIN) modulates Sprouty protein repression of mitogen-activated protein kinase (MAPK) activation by growth factor stimulation. J Biol Chem 287:13674-85
Sathyanarayana, Pradeep; Dev, Arvind; Pradeep, Anamika et al. (2012) Spry1 as a novel regulator of erythropoiesis, EPO/EPOR target, and suppressor of JAK2. Blood 119:5522-31
Collins, Sam; Waickman, Adam; Basson, Albert et al. (2012) Regulation of CD4? and CD8? effector responses by Sprouty-1. PLoS One 7:e49801
Kim, M K-H; Min, D J; Rabin, M et al. (2011) Functional characterization of Wilms tumor-suppressor WTX and tumor-associated mutants. Oncogene 30:832-42
Kuracha, Murali R; Burgess, Daniel; Siefker, Ed et al. (2011) Spry1 and Spry2 are necessary for lens vesicle separation and corneal differentiation. Invest Ophthalmol Vis Sci 52:6887-97
Michos, Odyssé; Cebrian, Cristina; Hyink, Deborah et al. (2010) Kidney development in the absence of Gdnf and Spry1 requires Fgf10. PLoS Genet 6:e1000809
Akbulut, Simge; Reddi, Alagarsamy L; Aggarwal, Priya et al. (2010) Sprouty proteins inhibit receptor-mediated activation of phosphatidylinositol-specific phospholipase C. Mol Biol Cell 21:3487-96
Shea, Kelly L; Xiang, Wanyi; LaPorta, Vincent S et al. (2010) Sprouty1 regulates reversible quiescence of a self-renewing adult muscle stem cell pool during regeneration. Cell Stem Cell 6:117-29

Showing the most recent 10 out of 26 publications