The overal goal of the work is to develop and test novel genetic methods to test the hypothesis that triple helix forming oligonucleotides can be used to deliver site-selective DNA damage and mutation in mammalian cells.
The specific aims of the work are to develop new models to study triple helix- mediated mutagenesis (based upon shuttle vector technology), to investigate the physical parameters which affect targeted mutagenesis upon these shuttle vectors in vitro, to examine DNA damage and repair pathways which influence TFO mediated mutation, and finally to extend the work to study TFO mediated mutation in chromosomal genes of the mouse, rather than shuttle vectors.
| Chin, Joanna Y; Glazer, Peter M (2009) Repair of DNA lesions associated with triplex-forming oligonucleotides. Mol Carcinog 48:389-99 |
| Lonkar, Pallavi; Kim, Ki-Hyun; Kuan, Jean Y et al. (2009) Targeted correction of a thalassemia-associated beta-globin mutation induced by pseudo-complementary peptide nucleic acids. Nucleic Acids Res 37:3635-44 |
| Schleifman, Erica B; Chin, Joanna Y; Glazer, Peter M (2008) Triplex-mediated gene modification. Methods Mol Biol 435:175-90 |
| Chin, Joanna Y; Kuan, Jean Y; Lonkar, Pallavi S et al. (2008) Correction of a splice-site mutation in the beta-globin gene stimulated by triplex-forming peptide nucleic acids. Proc Natl Acad Sci U S A 105:13514-9 |
| Kim, Ki-Hyun; Nielsen, Peter E; Glazer, Peter M (2007) Site-directed gene mutation at mixed sequence targets by psoralen-conjugated pseudo-complementary peptide nucleic acids. Nucleic Acids Res 35:7604-13 |
| Chin, Joanna Y; Schleifman, Erica B; Glazer, Peter M (2007) Repair and recombination induced by triple helix DNA. Front Biosci 12:4288-97 |
| Knauert, Melissa P; Kalish, Jennifer M; Hegan, Denise C et al. (2006) Triplex-stimulated intermolecular recombination at a single-copy genomic target. Mol Ther 14:392-400 |
| Knauert, Melissa P; Lloyd, Janice A; Rogers, Faye A et al. (2005) Distance and affinity dependence of triplex-induced recombination. Biochemistry 44:3856-64 |
| Kalish, Jennifer M; Seidman, Michael M; Weeks, Daniel L et al. (2005) Triplex-induced recombination and repair in the pyrimidine motif. Nucleic Acids Res 33:3492-502 |
| Rogers, Faye A; Manoharan, Muthiah; Rabinovitch, Peter et al. (2004) Peptide conjugates for chromosomal gene targeting by triplex-forming oligonucleotides. Nucleic Acids Res 32:6595-604 |
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