The principal investigator reports that azinomycins A and B are antitumor-antibiotic agents that were isolated from culture broths of Streptomyces griseofuscus and that these agents possess an intricately functionalized structure that contains the unprecedented aziridino(1,2- a)pyrrolidine ring system. It is noted that azinomycins A and B exhibit potent in vitro cytotoxic activity against L5178Y cells and significant in vivo antitumor activity against P388 leukemia in mice and that the agents have been shown to covalently cross-link DNA presumably via the electrophilic aziridine and epoxide systems, in a manner comparable to the clinically important antitumor agent mitomycin C. It is stated that the specific aims of this proposal are to develop methodology for the total synthesis of the antitumor agent azinomycin A, using new methodology specifically designed to deal with the relevant synthetic issue. The principal investigator also notes that he proposes to explore the structure/function relationships of the agents, in studies designed to define their molecular mechanism of interaction with oligonucleotides. He states that his research plan will involve the development of new strategies for the synthesis of substituted naphthalene ring systems, an enantioselective approach to the epoxide fragment, the development of functionalized phosphonates for introduction of the dehydroamino acid double bond, studies on the stereocontrolled synthesis of the 1- azabicyclo(3.1.0)hexane ring system, including effective methods for introduction of the differentiated 1,2-diol, and methods for convergent coupling of the individual fragments of the azinomycins, where the reactive aziridino(1,2-a)pyrrolidine is introduced as the final synthetic operation.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA065875-04
Application #
2414363
Study Section
Medicinal Chemistry Study Section (MCHA)
Project Start
1995-05-01
Project End
1998-07-31
Budget Start
1997-05-01
Budget End
1998-07-31
Support Year
4
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Ohio State University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210
Shi, Wei; Coleman, Robert S; Lowary, Todd L (2009) Synthesis and DNA-binding affinity studies of glycosylated intercalators designed as functional mimics of the anthracycline antibiotics. Org Biomol Chem 7:3709-22
Coleman, Robert S; Tierney, Mark T; Cortright, Sarah B et al. (2007) Synthesis of functional ""top-half"" partial structures of azinomycin a and B. J Org Chem 72:7726-35
Coleman, Robert S; Woodward, Robert L; Hayes, Amy M et al. (2007) Dependence of DNA sequence selectivity and cell cytotoxicity on azinomycin A and B epoxyamide stereochemistry. Org Lett 9:1891-4
Kelly, Gilbert T; Liu, Chaomin; Smith 3rd, Roger et al. (2006) Cellular effects induced by the antitumor agent azinomycin B. Chem Biol 13:485-92
Alcaro, Stefano; Ortuso, Francesco; Coleman, Robert S (2005) Molecular modeling of DNA cross-linking analogues based on the azinomycin scaffold. J Chem Inf Model 45:602-9
Alcaro, Stefano; Ortuso, Francesco; Coleman, Robert S (2002) DNA cross-linking by azinomycin B: Monte Carlo simulations in the evaluation of sequence selectivity. J Med Chem 45:861-70
Coleman, Robert S; Perez, Ronelito J; Burk, Christopher H et al. (2002) Studies on the mechanism of action of azinomycin B: definition of regioselectivity and sequence selectivity of DNA cross-link formation and clarification of the role of the naphthoate. J Am Chem Soc 124:13008-17
Coleman, Robert S; Burk, Christopher H; Navarro, Antonio et al. (2002) Role of the azinomycin naphthoate and central amide in sequence-dependent DNA alkylation and cytotoxicity of epoxide-bearing substructures. Org Lett 4:3545-8
Coleman, R S; Chen, W (2001) A convergent approach to the mitomycin ring system. Org Lett 3:1141-4
Coleman, R S; Garg, R (2001) Stereocontrolled synthesis of the diene and triene macrolactones of oximidines I and II: organometallic coupling versus standard macrolactonization. Org Lett 3:3487-90

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