This application from proposes to standardize measurements of differentiation-, proliferation-, and cell cycle-phase related and regulatory antigens in growing cells, as a means of extending the utility of the DNA content measurements now used for clinical and research purposes to characterize growing cell populations. The differentiation related markers, used to identify different histologic subpopulations, include intermediate filaments, and the proliferation markers include PCNA. Cyclins D, E, and B1 and P105 will be examined as cell cycle-related antigens, and products of genes such as myc, ras, and neu will be studied as regulatory proteins. Standardization will be accomplished by both biochemical analysis and by use of secondary standards in the form of fluorescent or antibody-binding beads. Additional studies will be carried out in collaboration with the University of Rochester and Los Alamos National Laboratory to determine whether the addition, respectively, of morphologic information derived from slit-scanning or of fluorescence lifetime information determined using a phase-sensitive (in this case referring to the phase of light waves, not cell cycle phase) flow cytometer improves detection sensitivity.
| Weis, Michael C; Avva, Jayant; Jacobberger, James W et al. (2014) A data-driven, mathematical model of mammalian cell cycle regulation. PLoS One 9:e97130 |
| Jacobberger, James W; Avva, Jayant; Sreenath, Sree N et al. (2012) Dynamic epitope expression from static cytometry data: principles and reproducibility. PLoS One 7:e30870 |
| Avva, Jayant; Weis, Michael C; Sramkoski, R Michael et al. (2012) Dynamic expression profiles from static cytometry data: component fitting and conversion to relative, ""same scale"" values. PLoS One 7:e38275 |
| Jacobberger, James W; Sramkoski, R Michael; Stefan, Tammy (2011) Multiparameter cell cycle analysis. Methods Mol Biol 699:229-49 |
| Stefan, Tammy; Jacobberger, James W (2011) Laser scanning cytometry of mitosis: state and stage analysis. Methods Cell Biol 102:341-72 |
| Singhania, Rajat; Sramkoski, R Michael; Jacobberger, James W et al. (2011) A hybrid model of mammalian cell cycle regulation. PLoS Comput Biol 7:e1001077 |
| Frisa, Phyllis S; Jacobberger, James W (2010) Cytometry of chromatin bound Mcm6 and PCNA identifies two states in G1 that are separated functionally by the G1 restriction point. BMC Cell Biol 11:26 |
| Frisa, Phyllis S; Jacobberger, James W (2009) Cell cycle-related cyclin b1 quantification. PLoS One 4:e7064 |
| Mailankot, Maneesh; Smith, Dawn; Howell, Scott et al. (2008) Cell cycle arrest by kynurenine in lens epithelial cells. Invest Ophthalmol Vis Sci 49:5466-75 |
| Soni, Deena V; Jacobberger, James W (2004) Inhibition of cdk1 by alsterpaullone and thioflavopiridol correlates with increased transit time from mid G2 through prophase. Cell Cycle 3:349-57 |
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