This competing renewal of CA119171, Nutrition and Physical Activity Assessment Study (NPAAS), continues to focus the use of biomarkers and regression calibration models to understand better the relationship between diet, physical activity and chronic disease risk in postmenopausal women. Our work uses objective biomarkers in regression calibration models to correct self-report data for random and systematic measurement biases. This work has provided evidence for strong positive associations of energy intake, and strong inverse associations of activity-related energy expenditure (AREE), with chronic disease risk; these associations were absent without measurement error correction. To date, suitable quantitative biomarkers have been available for few diet components (e.g. total energy, protein, potassium, sodium) and physical activity (AREE). For this grant funding period, we will extend our work by using innovative metabolomics profiling in blood and urine specimens to characterize food and nutrient intake. Metabolomics offers the possibility to advance the field of nutritional epidemiology in a manner not been possible with individual biomarkers since metabolomics uniquely accounts for dietary exposures in an integrated manner and explains > 50% of the variation in nutrient intake.
Our specific aims are: (1) To obtain serum and 24-hour urine metabolomic profiles for n=450 WHI-NPAAS participants who have stored blood and urine specimens from a previous project cycle. To use these profiles to calculate intake values for each nutritional variable for which a biomarker is established in our recently completed NPAAS feeding study. To use the resulting biomarker-based intake values to develop calibration equations for each nutritional variable using previously collected food frequency questionnaire, 4-day food record and 24-hour dietary recalls, as well as participant characteristics, from these 450 women. (2) To use the calibration equations developed in Aim 1 to estimate intake throughout WHI cohorts for each nutritional variable with suitable biomarkers. To conduct a series of analyses examining the association of metabolomics- calibrated nutrient intake with risk of major cancers, cardiovascular diseases and diabetes in WHI cohorts, where self-reported dietary intake and other key data are available at baseline for 161,000 postmenopausal women. (3) To use serum and spot urine metabolomic profiles to directly estimate intake for each nutritional variable having a suitable biomarker, in subset of about 12,000 WHI women with both stored blood and urine. Profiles will be obtained for n=708 adjudicated cancers (breast, colorectal, ovarian and endometrial) and n=708 controls. These analyses will assess novel nutrition and metabolite cancer associations without use of dietary self-report data. (4) To examine blood- and urine-based metabolomic profiles more broadly in relation to cancer risk in the Aim 3 case-control sample without regard to the role of specific metabolites in nutrient biomarker specification. Our highly productive research team is expected to make additional, innovative contributions in nutritional epidemiology and more broadly in chronic disease prevention research.
Dietary habits play a critical role in maintaining health and preventing chronic diseases such as cancer, cardiovascular disease and diabetes. However, inconsistent evidence from prior studies has prevented broad public health campaigns, patient interventions and policy initiatives from moving forward in a uniform manner. One reason for the lack of progress is that the tools and methods to assess diet and physical activity exposures are poor; objective biomarkers of diet from biological specimens may be an improvement. In this study we will use metabolomics profiling of blood and urine as markers of diet and will examine their association with cancer, cardiovascular disease and diabetes in postmenopausal women.
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|Rossouw, Jacques E; Prentice, Ross L (2018) Associations of fats and carbohydrates with cardiovascular disease and mortality-PURE and simple? Lancet 391:1677|
|Yun, Hee Young; Lampe, Johanna W; Tinker, Lesley F et al. (2018) Serum Nitrogen and Carbon Stable Isotope Ratios Meet Biomarker Criteria for Fish and Animal Protein Intake in a Controlled Feeding Study of a Women's Health Initiative Cohort. J Nutr 148:1931-1937|
|Neuhouser, Marian L (2018) The importance of healthy dietary patterns in chronic disease prevention. Nutr Res :|
|Tasevska, Natasha; Pettinger, Mary; Kipnis, Victor et al. (2018) Associations of Biomarker-Calibrated Intake of Total Sugars With the Risk of Type 2 Diabetes and Cardiovascular Disease in the Women's Health Initiative Observational Study. Am J Epidemiol 187:2126-2135|
|Freedman, Laurence S; Commins, John M; Willett, Walter et al. (2017) Evaluation of the 24-Hour Recall as a Reference Instrument for Calibrating Other Self-Report Instruments in Nutritional Cohort Studies: Evidence From the Validation Studies Pooling Project. Am J Epidemiol 186:73-82|
|Lampe, Johanna W; Huang, Ying; Neuhouser, Marian L et al. (2017) Dietary biomarker evaluation in a controlled feeding study in women from the Women's Health Initiative cohort. Am J Clin Nutr 105:466-475|
|Yetley, Elizabeth A; MacFarlane, Amanda J; Greene-Finestone, Linda S et al. (2017) Options for basing Dietary Reference Intakes (DRIs) on chronic disease endpoints: report from a joint US-/Canadian-sponsored working group. Am J Clin Nutr 105:249S-285S|
|Prentice, Ross L; Huang, Ying; Neuhouser, Marian L et al. (2017) Associations of Biomarker-Calibrated Sodium and Potassium Intakes With Cardiovascular Disease Risk Among Postmenopausal Women. Am J Epidemiol 186:1035-1043|
|Song, Xiaoling; Huang, Ying; Neuhouser, Marian L et al. (2017) Dietary long-chain fatty acids and carbohydrate biomarker evaluation in a controlled feeding study in participants from the Women's Health Initiative cohort. Am J Clin Nutr 105:1272-1282|
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