Abstract

The roles of reactive oxygen species (ROS), nitric oxide (NO), and cochlear blood flow in cochlear function and in sound-induced hearing loss will be examined with a combination of experimental manipulations and biochemical and physiological measurements.
In Aim 1, generation of ROS during and after ischemia will be evaluated by manipulating glutathione levels, measuring NO levels, blocking NO synthesis, and chelating iron (to diminish production of hydroxyl radical).
In Aim 2, roles of these factors in sound-induced hearing loss will be evaluated by examining the effects of similar manipulations on short-term and long-term hearing loss after high-level exposure to sound, and by hemodilution to alter blood flow and oxygen delivery.
In Aim 3, a newly-discovered trigeminal C-fiber innervation of the cochlear vasculature will be studied by electrical stimulation, observing the effects of on blood flow and cochlear vascular permeability, and by tract-tracing to establish more completely its anatomical path.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC000105-27
Application #
6379161
Study Section
Hearing Research Study Section (HAR)
Program Officer
Cooper, Judith
Project Start
1977-05-01
Project End
2002-08-31
Budget Start
2001-09-01
Budget End
2002-08-31
Support Year
27
Fiscal Year
2001
Total Cost
$410,661
Indirect Cost

  Institution

Name
Oregon Health and Science University
Department
Otolaryngology
Type
Schools of Medicine
DUNS #
009584210
City
Portland
State
OR
Country
United States
Zip Code
97239

  Publications

Wu, Tao; Ramamoorthy, Sripriya; Wilson, Teresa et al. (2016) Optogenetic Control of Mouse Outer Hair Cells. Biophys J 110:493-502
Warren, Rebecca L; Ramamoorthy, Sripriya; Ciganovi?, Nikola et al. (2016) Minimal basilar membrane motion in low-frequency hearing. Proc Natl Acad Sci U S A 113:E4304-10
Wilson, Teresa; Omelchenko, Irina; Foster, Sarah et al. (2014) JAK2/STAT3 inhibition attenuates noise-induced hearing loss. PLoS One 9:e108276
Shi, Xiaorui; Zhang, Fei; Urdang, Zachary et al. (2014) Thin and open vessel windows for intra-vital fluorescence imaging of murine cochlear blood flow. Hear Res 313:38-46
Floc'h, Johann Le; Tan, Winston; Telang, Ravindra S et al. (2014) Markers of cochlear inflammation using MRI. J Magn Reson Imaging 39:150-61
Reif, Roberto; Zhi, Zhongwei; Dziennis, Suzan et al. (2013) Changes in cochlear blood flow in mice due to loud sound exposure measured with Doppler optical microangiography and laser Doppler flowmetry. Quant Imaging Med Surg 3:235-42
Han, Wei-ju; Shi, Xiao-rui; Nuttall, Alfred (2013) Noise-induced nitrotyrosine increase and outer hair cell death in guinea pig cochlea. Chin Med J (Engl) 126:2923-7
Reif, Roberto; Qin, Jia; Shi, Lei et al. (2012) Monitoring hypoxia induced changes in cochlear blood flow and hemoglobin concentration using a combined dual-wavelength laser speckle contrast imaging and Doppler optical microangiography system. PLoS One 7:e52041
Dziennis, Suzan; Reif, Roberto; Zhi, Zhongwei et al. (2012) Effects of hypoxia on cochlear blood flow in mice evaluated using Doppler optical microangiography. J Biomed Opt 17:106003
Dai, Min; Shi, Xiaorui (2011) Fibro-vascular coupling in the control of cochlear blood flow. PLoS One 6:e20652

Showing the most recent 10 out of 54 publications