Meniere's Disease is one of the pathological entities characterized by endolymphatic hydrops of the cochlear and vestibular labyrinths. Hydrops can result from an alteration of ion transport properties of the epithelial cells bordering the endolymphatic system. Little is known about the cellular basis of the pathologic processes involved because data are lacking from normal as well as pathological systems concerning mechanisms of active secretion and absorption of ions and for ionic permeabilities. Endolymph Is unique in that it is the only extracellular fluid In the body with a high potassium concentration and low sodium concentration. This project will continue our study of the ion transport processes responsible for secretion of potassium and absorption of sodium in the vestibular labyrinth and cochlea by further utilizing our in vitro techniques. Specific parameters to be measured include transepithelial voltage and resistance, intracellular voltage and cell input resistance, luminal (apical) Ionic activities, cell volume (height) and currents through single ionic channels. The membrane characteristics will be deduced from the observed changes in the above parameters due to experimental changes in the composition of the solutions (including application of experimental and ototoxic drugs) in contact with the apical and basolateral cell membranes.

National Institute of Health (NIH)
National Institute on Deafness and Other Communication Disorders (NIDCD)
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Hearing Research Study Section (HAR)
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Father Flanagan's Boys' Home
Boys Town
United States
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Kudo, Takayuki; Wangemann, Philine; Marcus, Daniel C (2018) Claudin expression during early postnatal development of the murine cochlea. BMC Physiol 18:1
Miyazaki, Hiromitsu; Wangemann, Philine; Marcus, Daniel C (2016) The gastric H,K-ATPase in stria vascularis contributes to pH regulation of cochlear endolymph but not to K secretion. BMC Physiol 17:1
Kim, Kyunghee X; Sanneman, Joel D; Kim, Hyoung-Mi et al. (2014) Slc26a7 chloride channel activity and localization in mouse Reissner's membrane epithelium. PLoS One 9:e97191
Pondugula, Satyanarayana R; Kampalli, Suresh B; Wu, Tao et al. (2013) cAMP-stimulated Cl- secretion is increased by glucocorticoids and inhibited by bumetanide in semicircular canal duct epithelium. BMC Physiol 13:6
Yoo, Jae Chul; Kim, Hye-Young; Han, Kyu-Hee et al. (2012) Na+ absorption by Claudius' cells is regulated by purinergic signaling in the cochlea. Acta Otolaryngol 132 Suppl 1:S103-8
Kim, Sung Huhn; Marcus, Daniel C (2011) Regulation of sodium transport in the inner ear. Hear Res 280:21-9
Yamazaki, Muneharu; Kim, Kyunghee X; Marcus, Daniel C (2011) Sodium selectivity of Reissner's membrane epithelial cells. BMC Physiol 11:4
Kim, Hyoung-Mi; Wangemann, Philine (2011) Epithelial cell stretching and luminal acidification lead to a retarded development of stria vascularis and deafness in mice lacking pendrin. PLoS One 6:e17949
Kim, Kyunghee X; Marcus, Daniel C (2010) Inward-rectifier chloride currents in Reissner's membrane epithelial cells. Biochem Biophys Res Commun 394:434-8
Kim, Hyoung-Mi; Wangemann, Philine (2010) Failure of fluid absorption in the endolymphatic sac initiates cochlear enlargement that leads to deafness in mice lacking pendrin expression. PLoS One 5:e14041

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