Abstract

Experiments are proposed to determine if the smallest odor-induced receptor currents can cause the olfactory receptor cell to generate an action potential. The resting electrical constants of the cell will be measured and corrected for leak current. Experiments will then attempt to establish that the secondary chloride current amplifies the receptor current without increasing the noise (variance) of the current in order to detect low concentrations of odors. The work proposed will advance the molecular and cellular processes which subserve sensory function.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC000926-06
Application #
2770204
Study Section
Sensory Disorders and Language Study Section (CMS)
Project Start
1993-07-01
Project End
2000-08-31
Budget Start
1998-09-01
Budget End
1999-08-31
Support Year
6
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Cincinnati
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
City
Cincinnati
State
OH
Country
United States
Zip Code
45221

  Publications

Badamdorj, Dorjsuren; Edwards, David A; French, Donald A et al. (2008) Identification of Cl(Ca) channel distributions in olfactory cilia. Math Methods Appl Sci 31:1860-1873
Smith, David W; Thach, Sokunthirith; Marshall, Erika L et al. (2008) Mice lacking NKCC1 have normal olfactory sensitivity. Physiol Behav 93:44-9
Nickell, William T; Kleene, Nancy K; Kleene, Steven J (2007) Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium. J Physiol 583:1005-20
French, Donald A; Flannery, Richard J; Groetsch, Charles W et al. (2006) Numerical Approximation of Solutions of a Nonlinear Inverse Problem Arising in Olfaction Experimentation. Math Comput Model 43:945-956
Nickell, William T; Kleene, Nancy K; Gesteland, Robert C et al. (2006) Neuronal chloride accumulation in olfactory epithelium of mice lacking NKCC1. J Neurophysiol 95:2003-6
Flannery, Richard J; French, Donald A; Kleene, Steven J (2006) Clustering of cyclic-nucleotide-gated channels in olfactory cilia. Biophys J 91:179-88
Pun, Raymund Y K; Kleene, Steven J (2004) An estimate of the resting membrane resistance of frog olfactory receptor neurones. J Physiol 559:535-42
Pun, Raymund Y K; Kleene, Steven J (2003) Contribution of cyclic-nucleotide-gated channels to the resting conductance of olfactory receptor neurons. Biophys J 84:3425-35
Pun, Raymund Y K; Kleene, Steven J (2002) Outward currents in olfactory receptor neurons activated by odorants and by elevation of cyclic AMP. Cell Biochem Biophys 37:15-26
Kleene, S J (2000) Spontaneous gating of olfactory cyclic-nucleotide-gated channels. J Membr Biol 178:49-54

Showing the most recent 10 out of 18 publications