The primary olfactory centers in the brains of diverse animals, including humans, are characterized by an array of synaptic modules called glomeruli. These centers are thought to be organized chemotopically, such that odor information is represented spatially among glomeruli, but this important hypothesis has not been tested comprehensively in a species offering the advantages of anatomical simplicity, identifiable glomeruli, accessible receptor cells and central neurons, and chemically identified, behaviorally relevant odors. Moreover, despite dramatic advances in chemosensory research, we still do not understand how complex odor stimuli are encoded in neural activity, within and among glomeruli, that ultimately leads to appropriate behavioral responses. This project builds on a firm foundation of technical experience and knowledge about an experimentally favorable model, the olfactory system of Manduca sexta, which is comparable to its vertebrate counterpart in organization and function and permits the hypothesis of glomerular chemotopy to be tested with greater precision than has been possible in other species. This model system also offers an exceptional opportunity to unravel the synaptic neural circuitry within and between identified glomeruli in order to reveal how specific odor information is processed at its first way-station in the brain. By means of intracellular recording and staining, extracellular multichannel recording, laser-scanning confocal and transmission electron microscopy, and computer-assisted mathematical modeling, we will focus on identified glomeruli in recognizable clusters to: (1) test the hypothesis that glomeruli are organized chemotopically by determining what chemical, temporal, and intensity properties of behaviorally significant odor stimuli are analyzed and encoded by individual neurons innervating particular glomeruli; (2) characterize synaptic circuits within and between glomeruli to learn how the various types of neurons associated with glomeruli interact to shape the signas conveyed by output neurons projecting to higher centers in the brain; and (3) develop mathematical models of characterized neurons to generate testable hypotheses about their physiology and synaptic interactions. This research will promote understanding of basic olfactory mechanisms in all animals, including mankind, and promises to contribute toward explanation of sensory disorders such as parosmia, hyposmia, and anosmia.
| Goldman-Huertas, Benjamin; Mitchell, Robert F; Lapoint, Richard T et al. (2015) Evolution of herbivory in Drosophilidae linked to loss of behaviors, antennal responses, odorant receptors, and ancestral diet. Proc Natl Acad Sci U S A 112:3026-31 |
| Reisenman, Carolina E (2014) Hunger is the best spice: effects of starvation in the antennal responses of the blood-sucking bug Rhodnius prolixus. J Insect Physiol 71:8-13 |
| Lei, Hong; Chiu, Hong-Yan; Hildebrand, John G (2013) Responses of protocerebral neurons in Manduca sexta to sex-pheromone mixtures. J Comp Physiol A Neuroethol Sens Neural Behav Physiol 199:997-1014 |
| Martin, Joshua P; Lei, Hong; Riffell, Jeffrey A et al. (2013) Synchronous firing of antennal-lobe projection neurons encodes the behaviorally effective ratio of sex-pheromone components in male Manduca sexta. J Comp Physiol A Neuroethol Sens Neural Behav Physiol 199:963-79 |
| Riffell, Jeffrey A; Lei, Hong; Abrell, Leif et al. (2013) Neural basis of a pollinator's buffet: olfactory specialization and learning in Manduca sexta. Science 339:200-4 |
| Heinbockel, Thomas; Shields, Vonnie D C; Reisenman, Carolina E (2013) Glomerular interactions in olfactory processing channels of the antennal lobes. J Comp Physiol A Neuroethol Sens Neural Behav Physiol 199:929-46 |
| Martin, Joshua P; Beyerlein, Aaron; Dacks, Andrew M et al. (2011) The neurobiology of insect olfaction: sensory processing in a comparative context. Prog Neurobiol 95:427-47 |
| Reisenman, Carolina E; Dacks, Andrew M; Hildebrand, John G (2011) Local interneuron diversity in the primary olfactory center of the moth Manduca sexta. J Comp Physiol A Neuroethol Sens Neural Behav Physiol 197:653-65 |
| Lei, Hong; Reisenman, Carolina E; Wilson, Caroline H et al. (2011) Spiking patterns and their functional implications in the antennal lobe of the tobacco hornworm Manduca sexta. PLoS One 6:e23382 |
| Reisenman, Carolina E; Riffell, Jeffrey A; Bernays, Elizabeth A et al. (2010) Antagonistic effects of floral scent in an insect-plant interaction. Proc Biol Sci 277:2371-9 |
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