Abstract

This research is aimed at describing in isolated osteroclasts the cytoskeletal components, the subcellular location and function of several enzymes, the elemental composition, and the mitochondrial activity. These aspects of cell biology will be examined in different hormonal environments. To accomplish this we will employ the techniques of indirect immunofluorescent antibody microscopy, electron microprobe analysis, and fluorescence microscopy. The several aspects of osteoclast structure, composition and function we propose to investigate should provide further information for establishing a more rational basis in designing therapeutic measures directed at metabolic diseases of the skeleton.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE004345-10
Application #
3219036
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1976-04-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
10
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Pennsylvania State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
University Park
State
PA
Country
United States
Zip Code
16802

  Publications

Brubaker, K D; Mao, F; Gay, C V (1999) Localization of carbonic anhydrase in living osteoclasts with bodipy 558/568-modified acetazolamide, a thiadiazole carbonic anhydrase inhibitor. J Histochem Cytochem 47:545-50
Brubaker, K D; Gay, C V (1999) Evidence for plasma membrane-mediated effects of estrogen. Calcif Tissue Int 64:459-62
Brubaker, K D; Gay, C V (1999) Depolarization of osteoclast plasma membrane potential by 17beta-estradiol. J Bone Miner Res 14:1861-6
Brubaker, K D; Gay, C V (1999) Estrogen stimulates protein tyrosine phosphorylation and Src kinase activity in avian osteoclasts. J Cell Biochem 76:206-16
Hunter, S J; Gay, C V; Osdoby, P A et al. (1998) Spectrin localization in osteoclasts: immunocytochemistry, cloning, and partial sequencing. J Cell Biochem 71:204-15
May, L G; Gay, C V (1997) Multiple G-protein involvement in parathyroid hormone regulation of acid production by osteoclasts. J Cell Biochem 64:161-70
May, L G; Gay, C V (1997) Parathyroid hormone uses both adenylate cyclase and protein kinase C to regulate acid production in osteoclasts. J Cell Biochem 65:565-73
Gay, C V (1996) Role of microscopy in elucidating the mechanism and regulation of the osteoclast resorptive apparatus. Microsc Res Tech 33:165-70
May, L G; Gay, C V (1996) Development of a new method for obtaining osteoclasts from endosteal surfaces. In Vitro Cell Dev Biol Anim 32:269-78
Brubaker, K D; Gay, C V (1994) Specific binding of estrogen to osteoclast surfaces. Biochem Biophys Res Commun 200:899-907

Showing the most recent 10 out of 37 publications