Abstract

The formation of dentin by odontoblast involves the initial deposition of an uncalcified matrix, predentin that is transformed to dentin several microns from the cell borders. The biochemical mechanisms involved in this process are unknown, but certain data suggest that noncollagenous proteins (NCPs) and proteoglycans (PGs) are involved.
The aim of these investigations is to elucidate the nature, metabolism and biological significance of NCPs and PGs of rat dentin in order to ascertain the role they might play in dentinogenesis. In recent studies from this laboratory we have employed improved techniques to isolate these macromolecules with procedures designed to eliminate proteolytic degradation and losses. These studies have shown that rat dentin contains phosphoproteins, two pools of PGs, acidic glycoproteins, serum proteins and Gamma-carboxyglutamate (G1a)- containing proteins. Each class of macromolecules is being further characterized biochemically. An improved odontoblast organ culture system is being used to study the biosynthesis of the NCPs and PGs. These studies are designed to advance our knowledge about the basic mechanisms involved in the formation of teeth and bones.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE005092-08
Application #
3219237
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1979-03-01
Project End
1987-02-28
Budget Start
1986-03-01
Budget End
1987-02-28
Support Year
8
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Alabama Birmingham
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294

  Publications

Liang, Tian; Meng, Tian; Wang, Suzhen et al. (2016) The LPV Motif Is Essential for the Efficient Export of Secretory DMP1 From the Endoplasmic Reticulum. J Cell Physiol 231:1468-75
Gibson, Monica Prasad; Jani, Priyam; Wang, Xiaofang et al. (2014) Overexpressing the NH2-terminal fragment of dentin sialophosphoprotein (DSPP) aggravates the periodontal defects in Dspp knockout mice. J Oral Biosci 56:143-148
Gibson, Monica Prasad; Jani, Priyam; Liu, Ying et al. (2013) Failure to process dentin sialophosphoprotein into fragments leads to periodontal defects in mice. Eur J Oral Sci 121:545-50
Zurick, Kevin M; Qin, Chunlin; Bernards, Matthew T (2013) Mineralization induction effects of osteopontin, bone sialoprotein, and dentin phosphoprotein on a biomimetic collagen substrate. J Biomed Mater Res A 101:1571-81
Sun, Yao; Jiang, Yong; Liu, Qilin et al. (2013) Biomimetic engineering of nanofibrous gelatin scaffolds with noncollagenous proteins for enhanced bone regeneration. Tissue Eng Part A 19:1754-63
Gibson, M P; Zhu, Q; Liu, Q et al. (2013) Loss of dentin sialophosphoprotein leads to periodontal diseases in mice. J Periodontal Res 48:221-7
Liu, Q; Gibson, M P; Sun, Hongchen et al. (2013) Dentin sialophosphoprotein (DSPP) plays an essential role in the postnatal development and maintenance of mouse mandibular condylar cartilage. J Histochem Cytochem 61:749-58
Li, Changcheng; Xie, Xiaohua; Wang, Xiaofang et al. (2013) Differential expression and localization of dentin matrix protein 1 (DMP1) fragments in mouse submandibular glands. J Mol Histol 44:231-9
Gibson, Monica P; Liu, Qilin; Zhu, Qinglin et al. (2013) Role of the NH2 -terminal fragment of dentin sialophosphoprotein in dentinogenesis. Eur J Oral Sci 121:76-85
Gibson, Monica Prasad; Zhu, Qinglin; Wang, Suzhen et al. (2013) The rescue of dentin matrix protein 1 (DMP1)-deficient tooth defects by the transgenic expression of dentin sialophosphoprotein (DSPP) indicates that DSPP is a downstream effector molecule of DMP1 in dentinogenesis. J Biol Chem 288:7204-14

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