The present research proposal aims to expand upon substantial initial progress made toward clarifying the biochemical ontogeny of development of the secondary palate. Differentiation of the palate may be defined in terms of glycosaminoglycan, collagen, cyclic nucleotide and prostaglandin synthesis. Beta-adrenergic receptor occupancy also appears to play a significant role in palatal development. We, therefore, propose (1) to characterize the Beta-adrenergic receptor system and a prostaglandin receptor system in the developing murine secondary palate by receptor-radioactive ligand competitive binding assays; (2) investigate factors regulating properties of these receptor systems in vitro using primarily cAMP radioimmunoassay; (3) determine involvement of catecholamines in qualitative and quantitative synthesis of palatal extracellular glycosaminoglycans, collagen and cell proliferation using cellulose acetate and polyacrilamide gel electrophoresis and collagen types I and III radioimmunoassay; and (4) determine hormonal responsiveness of palatal epithelium following heterospecific epithelial-mesenchymal recombination.
| Mukhopadhyay, Partha; Greene, Robert M; Pisano, M Michele (2015) Cigarette smoke induces proteasomal-mediated degradation of DNA methyltransferases and methyl CpG-/CpG domain-binding proteins in embryonic orofacial cells. Reprod Toxicol 58:140-8 |
| Warner, Dennis R; Mukhopadhyay, Partha; Brock, Guy et al. (2014) MicroRNA expression profiling of the developing murine upper lip. Dev Growth Differ 56:434-47 |
| Mukhopadhyay, Partha; Brock, Guy; Appana, Savitri et al. (2011) MicroRNA gene expression signatures in the developing neural tube. Birth Defects Res A Clin Mol Teratol 91:744-62 |
| Warner, Dennis R; Mukhopadhyay, Partha; Brock, Guy N et al. (2011) TGF?-1 and Wnt-3a interact to induce unique gene expression profiles in murine embryonic palate mesenchymal cells. Reprod Toxicol 31:128-33 |
| Greene, Robert M; Pisano, M Michele (2010) Palate morphogenesis: Current understanding and future directions. Birth Defects Res C Embryo Today 90:133-54 |
| Mukhopadhyay, Partha; Rezzoug, Francine; Webb, Cynthia L et al. (2009) Suppression of chondrogenesis by Id helix-loop-helix proteins in murine embryonic orofacial tissue. Differentiation 77:462-72 |
| Warner, Dennis R; Smith, Henry S; Webb, Cynthia L et al. (2009) Expression of Wnts in the developing murine secondary palate. Int J Dev Biol 53:1105-12 |
| Mukhopadhyay, Partha; Webb, Cynthia L; Warner, Dennis R et al. (2008) BMP signaling dynamics in embryonic orofacial tissue. J Cell Physiol 216:771-9 |
| Yin, Xiaolong; Warner, Dennis R; Roberts, Emily A et al. (2005) Identification of novel CBP interacting proteins in embryonic orofacial tissue. Biochem Biophys Res Commun 329:1010-7 |
| Nugent, P; Greene, R M (1994) Interactions between the transforming growth factor beta (TGF beta) and retinoic acid signal transduction pathways in murine embryonic palatal cells. Differentiation 58:149-55 |
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