The dental follicle, a loose connective tissue sac which surrounds the tooth, is required for eruption, and along with the adjacent stellate reticulum (SR), contains genes coding for the probable eruption molecules. A cascade of molecular signals in which there is cross-talk between the follicle and SR may initiate the cellular events of eruption; namely, early postnatal recruitment of mononuclear cells to the follicle to form osteoclasts needed for bone resorption to form the eruption pathway. Thus, it is the objective of this proposal to determine if certain of these molecules present in vivo are required for eruption and what is the role of such molecules in initiating the cellular events. To that end, monocyte chemotactic protein-1 (MCP-1), a molecule present in the dental follicle, will be injected into rats to determine its effect on the time of eruption and its ability to recruit the mononuclear cells into the follicle. The ability of the follicle cells to secrete MCP-1 in response to other putative eruption molecules also will be determined. Two molecules present in the SR, interleukin-1alpha (IL-1alpha) and parathyroid hormone related protein (PTHrP), may signal molecules in the follicle to initiate eruption. Injections of these molecules, use of null mice devoid of the receptor gene for IL-1alpha, and analysis of gene expression by RT-PCR and ribonuclease protection assay techniques all will be used to determine if these molecules are necessary for eruption, as well as determining how they affect gene expression and mononuclear cell numbers. Gene expression and immunolocalization of interleukin-6 in the follicle also will be examined. By determining what local molecular signals are required for eruption, an understanding of why teeth sometimes do not erupt (e.g., third molars in humans) may be gained. In turn, molecular treatment may someday be used to induce impacted teeth to erupt, as well as perhaps initiate tooth movement that often is required in orthodontics. Additionally, because bone resorption is required for eruption, these studies also will provide answers about the molecular basis of alveolar bone resorption, an understanding of which is critical in treatment of periodontal diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE008911-09
Application #
6176800
Study Section
Oral Biology and Medicine Subcommittee 1 (OBM)
Project Start
1991-07-01
Project End
2003-04-05
Budget Start
2000-04-06
Budget End
2001-04-05
Support Year
9
Fiscal Year
2000
Total Cost
$168,830
Indirect Cost
Name
Louisiana State University A&M Col Baton Rouge
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
075050765
City
Baton Rouge
State
LA
Country
United States
Zip Code
70803
Yao, Shaomian; Li, Chunhong; Beckley, Michael et al. (2017) Expression of odontogenic ameloblast-associated protein in the dental follicle and its role in osteogenic differentiation of dental follicle stem cells. Arch Oral Biol 78:6-12
Rezai-Rad, Maryam; Bova, Jonathan F; Orooji, Mahdi et al. (2015) Evaluation of bone regeneration potential of dental follicle stem cells for treatment of craniofacial defects. Cytotherapy 17:1572-81
Rezai Rad, Maryam; Liu, Dawen; He, Hongzhi et al. (2015) The role of dentin matrix protein 1 (DMP1) in regulation of osteogenic differentiation of rat dental follicle stem cells (DFSCs). Arch Oral Biol 60:546-56
Yao, Shaomian; He, Hongzhi; Gutierrez, Dina L et al. (2013) Expression of bone morphogenetic protein-6 in dental follicle stem cells and its effect on osteogenic differentiation. Cells Tissues Organs 198:438-47
Rezai Rad, M; Wise, G E; Brooks, H et al. (2013) Activation of proliferation and differentiation of dental follicle stem cells (DFSCs) by heat stress. Cell Prolif 46:58-66
Liu, Dawen; Yao, Shaomian; Wise, Gary E (2012) Regulation of SFRP-1 expression in the rat dental follicle. Connect Tissue Res 53:366-72
Wise, Gary E; He, Hongzhi; Gutierrez, Dina L et al. (2011) Requirement of alveolar bone formation for eruption of rat molars. Eur J Oral Sci 119:333-8
Yao, Shaomian; Gutierrez, Dina L; Ring, Sherry et al. (2010) Electroporation to deliver plasmid DNA into rat dental tissues. J Gene Med 12:981-9
Liu, Dawen; Yao, Shaomian; Wise, Gary E (2010) MyD88 expression in the rat dental follicle: implications for osteoclastogenesis and tooth eruption. Eur J Oral Sci 118:333-41
Yao, Shaomian; Prpic, Veronica; Pan, Fenghui et al. (2010) TNF-alpha upregulates expression of BMP-2 and BMP-3 genes in the rat dental follicle--implications for tooth eruption. Connect Tissue Res 51:59-66

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