Periodontal diseases are one of the most common bacterial infections in developed countries. Although the identities of the bacteria responsible for the disease and the mechanisms of pathogenicity are understood to some degree, further insights into the molecular and cellular interactions between the pathogenic bacteria and the host are required before the disease can he brought under control. In this study, the interactions between Porphyromonas gingivalis, a periodontal pathogen, and gingival epithelial cells will be investigated. It has been established that P. gingivalis can invade primary cultures of gingival epithelial cells. The ultrastructural and biochemical requirements of P. gingivalis and of gingival epithelial cells that are necessary for invasion will be determined. A variety of inhibitors of epithelial cell functions, such as microfilament and microtubule formation, protein synthesis and energy metabolism, will be examined for their ability to inhibit the invasion process. Similarly, inhibitors of bacterial cell functions such as macromolecule synthesis and energy metabolism will be tested for their effects on invasion. Thus, the nature of the invasion process will be elucidated. The molecules of P. gingivalis that mediate adherence and induction of the invasion process will also be investigated. Surface molecules of P. gingivalis will be purified and examined for binding to epithelial cells. The genes for interactive molecules will be identified by cloning and transposon mutagenesis experiments. Finally, the physiology of the signal transduction, in particular calcium ion fluxes, that occurs in the epithelial cells subsequent to the initial interaction with P. gingivalis will be investigated. The information provided by these studies will enhance our understanding of how pathogenic bacteria and host cells interact during the disease process, thus facilitating the development of novel methods to control the disease.

National Institute of Health (NIH)
National Institute of Dental & Craniofacial Research (NIDCR)
Research Project (R01)
Project #
Application #
Study Section
Oral Biology and Medicine Subcommittee 1 (OBM)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Washington
Schools of Dentistry
United States
Zip Code
Armstrong, Cortney L; Klaes, Christopher K; Vashishta, Aruna et al. (2018) Filifactor alocis manipulates human neutrophils affecting their ability to release neutrophil extracellular traps induced by PMA. Innate Immun 24:210-220
Lee, Jae Y; Miller, Daniel P; Wu, Leng et al. (2018) Maturation of the Mfa1 Fimbriae in the Oral Pathogen Porphyromonas gingivalis. Front Cell Infect Microbiol 8:137
Sztukowska, Maryta N; Dutton, Lindsay C; Delaney, Christopher et al. (2018) Community Development between Porphyromonas gingivalis and Candida albicans Mediated by InlJ and Als3. MBio 9:
Lamont, Richard J; Koo, Hyun; Hajishengallis, George (2018) The oral microbiota: dynamic communities and host interactions. Nat Rev Microbiol 16:745-759
Inaba, Hiroaki; Amano, Atsuo; Lamont, Richard J et al. (2018) Cell Cycle Arrest and Apoptosis Induced by Porphyromonas gingivalis Require Jun N-Terminal Protein Kinase- and p53-Mediated p38 Activation in Human Trophoblasts. Infect Immun 86:
Edmisson, Jacob S; Tian, Shifu; Armstrong, Cortney L et al. (2018) Filifactor alocis modulates human neutrophil antimicrobial functional responses. Cell Microbiol 20:e12829
Kuboniwa, Masae; Houser, John R; Hendrickson, Erik L et al. (2017) Metabolic crosstalk regulates Porphyromonas gingivalis colonization and virulence during oral polymicrobial infection. Nat Microbiol 2:1493-1499
Miller, Daniel P; Hutcherson, Justin A; Wang, Yan et al. (2017) Genes Contributing to Porphyromonas gingivalis Fitness in Abscess and Epithelial Cell Colonization Environments. Front Cell Infect Microbiol 7:378
Jimenez Flores, E; Tian, S; Sizova, M et al. (2017) Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis. Infect Immun 85:
Gao, Shegan; Li, Shuoguo; Ma, Zhikun et al. (2016) Presence of Porphyromonas gingivalis in esophagus and its association with the clinicopathological characteristics and survival in patients with esophageal cancer. Infect Agent Cancer 11:3

Showing the most recent 10 out of 93 publications