Abstract

The objectives of this proposal are to examine the hypothesis that refractory disease is distinct from nonrefractory periodontal disease and to identify one or more well-defined syndromes within the refractory subjects based on analysis of microbial and host factors.
The specific aims are: 1) identify the predominant bacteria in refractory subjects including unrecognized and uncultivated species using 16S rRNA gene sequences. Analyzing 16S rDNA genes cloned from plaque libraries. Identify the uncultivated oral species and determine their role in periodontal disease. 2) Test the hypothesis that subjects with refractory disease can be distinguished from subjects with treatable periodontitis and periodontal health based upon microbial composition. Twenty-eight sites in 40 subjects in each of 3 groups will be analyzed for levels of 60 cultivable taxa and 120 uncultivated taxa using checkerboard hybridization assays. Allow identification of species that are useful for distinguishing between refractory and treatable periodontitis and health. 3) Test the hypothesis that subjects with refractory disease(s) can be distinguished from those with treatable disease and health based upon host factors such as, genotypes, allotypes, serum IgG2 levels and specific antibodies levels to 40 specific periodontal bacteria. 4) Identify more than 1 disease syndromes within the refractory group based upon microbial and host markers.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
3R01DE011443-04S1
Application #
6324011
Study Section
Oral Biology and Medicine Subcommittee 1 (OBM)
Program Officer
Mangan, Dennis F
Project Start
1997-04-01
Project End
2002-02-28
Budget Start
2000-03-01
Budget End
2002-02-28
Support Year
4
Fiscal Year
2000
Total Cost
$80,727
Indirect Cost

  Institution

Name
Forsyth Institute
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02142

  Publications

Albandar, Jasim M; Khattab, Razan; Monem, Fawza et al. (2012) The subgingival microbiota of Papillon-Lefevre syndrome. J Periodontol 83:902-8
Filkins, L M; Hampton, T H; Gifford, A H et al. (2012) Prevalence of streptococci and increased polymicrobial diversity associated with cystic fibrosis patient stability. J Bacteriol 194:4709-17
Colombo, Ana Paula V; Bennet, Susan; Cotton, Sean L et al. (2012) Impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray. J Periodontol 83:1279-87
Berlanga, Mercedes; Paster, Bruce J; Grandcolas, Philippe et al. (2011) Comparison of the gut microbiota from soldier and worker castes of the termite Reticulitermes grassei. Int Microbiol 14:83-93
Nossa, Carlos W; Oberdorf, William E; Yang, Liying et al. (2010) Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome. World J Gastroenterol 16:4135-44
Berlanga, Mercedes; Paster, Bruce J; Guerrero, Ricardo (2009) The taxophysiological paradox: changes in the intestinal microbiota of the xylophagous cockroach Cryptocercus punctulatus depending on the physiological state of the host. Int Microbiol 12:227-36
Preza, D; Olsen, I; Willumsen, T et al. (2009) Diversity and site-specificity of the oral microflora in the elderly. Eur J Clin Microbiol Infect Dis 28:1033-40
Colombo, Ana Paula V; Boches, Susan K; Cotton, Sean L et al. (2009) Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. J Periodontol 80:1421-32
Preza, D; Olsen, I; Willumsen, T et al. (2009) Microarray analysis of the microflora of root caries in elderly. Eur J Clin Microbiol Infect Dis 28:509-17
Paster, Bruce J; Dewhirst, Floyd E (2009) Molecular microbial diagnosis. Periodontol 2000 51:38-44

Showing the most recent 10 out of 36 publications