Essential to oral biofilm development is the initial colonization by oral streptococci. The abundant oral streptococci keep pathogens at bay. We have used the most abundant oral streptococcus, Streptococcus parasanguinis as a model to study bacterial colonization and identified a new family of bacterial serine-rich repeat proteins (SRRPs) named ?fimbriae-associated protein-1? (Fap1). Fap1 is heavily glycosylated, and glycosylation of Fap1 is crucial for bacterial biofilm formation. Since our discovery of Fap1, Fap1-like SRRPs have been identified from numerous Gram-positive bacteria and implicated in bacterial fitness and virulence. Our studies have led to the groundbreaking discovery of a new Fap1 biosynthetic pathway. We have demonstrated that the Fap1 biogenesis is controlled by a gene cluster encoding a series of novel glycosyltransferases and unique accessory secretion proteins. Biogenesis of SRRPs has now emerged as a new paradigm to investigate bacterial protein glycosylation and secretion. During our study of Fap1 glycosylation, we have defined a complete glycosylation pathway that synthesizes a novel Fap1 glycan. In the study of Fap1 secretion, we have identified a protein complex consisting of three distinct glycosylation associated proteins Gap1, 2 and 3 that work in concert to modulate the Fap1 maturation and biogenesis. Further, we have determined the high resolution 3-dimensional structure of the Gap1/2/3 complex, which uncovered new mechanistic insights for this 3-protein complex. Gap1 and Gap2 exhibit dual functions in the biogenesis of Fap1. 1), Gap1 and Gap2 modulates the formation of the protein complex as a molecular chaperone. 2), Gap1 and Gap2 function as a glucosyltransferase and glucosidase respectively in the quality control of Fap1 maturation and biogenesis. The Gap protein complex resembles the three-key elements in the eukaryotic quality control system dedicated to glycosylated proteins, hence we will continue our basic science discovery of new biology and biochemistry linked to maturation and biogenesis of Fap1 & other SRRPs.
Aim 1 Determine how Gap1 functions as a molecular chaperone for Gap2 and as a key quality control glycosyltransferase to process Fap1 precursor during Fap1 biogenesis. We will use genetic, biochemical, structural biology, and glycobiology approaches to investigate how Gap1 stabilizes Gap2 as a molecular chaperone, and how Gap1 acts as a quality control glycosyltransferase to process Fap1 precursor.
Aim 2 Define the roles played by Gap2 as a molecular chaperone for Gap3 and as a key quality control glucosidase during Fap1 biogenesis. We will determine how Gap2 assists Gap3 as an accessory chaperone, and coordinates with Gap1 and Gap3 to process Fap1, thus modulating the Fap1 biogenesis. As biogenesis of SRRPs is highly conserved in Gram-positive bacteria, deciphering novel molecular insight to this new protein complex as a quality control system will offer new opportunities to develop new strategies to maintain healthy oral cavity as well as to combat bacterial infections.

Public Health Relevance

Oral microbial homeostasis is not only important for oral health but also impacts systemic conditions. Our proposal investigates novel bacterial glycosylation and secretion mechanisms that are crucial for biofilm formation and bacterial virulence. The exciting basic science discovery from the application will reveal new targets that are amenable to device novel strategies to maintain a healthy oral environment, and keep pathogens at bay.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE017954-15
Application #
10300579
Study Section
Oral, Dental and Craniofacial Sciences Study Section (ODCS)
Program Officer
Mcnealy, Tamara Lyn
Project Start
2020-08-03
Project End
2023-02-28
Budget Start
2021-03-01
Budget End
2022-02-28
Support Year
15
Fiscal Year
2021
Total Cost
Indirect Cost
Name
Oregon Health and Science University
Department
Dentistry
Type
Schools of Dentistry/Oral Hygn
DUNS #
096997515
City
Portland
State
OR
Country
United States
Zip Code
97239
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Zhang, Hua; Zhou, Meixian; Yang, Tiandi et al. (2016) New Helical Binding Domain Mediates a Glycosyltransferase Activity of a Bifunctional Protein. J Biol Chem 291:22106-22117

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