Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK007422-32
Application #
2134947
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1976-05-01
Project End
1997-11-30
Budget Start
1995-12-15
Budget End
1996-11-30
Support Year
32
Fiscal Year
1996
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Pharmacology
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Zhao, Q; Abeygunawardana, C; Mildvan, A S (1997) NMR studies of the secondary structure in solution and the steroid binding site of delta5-3-ketosteroid isomerase in complexes with diamagnetic and paramagnetic steroids. Biochemistry 36:3458-72
Zhao, Q; Abeygunawardana, C; Gittis, A G et al. (1997) Hydrogen bonding at the active site of delta 5-3-ketosteroid isomerase. Biochemistry 36:14616-26
Zhao, Q; Abeygunawardana, C; Mildvan, A S (1996) 13C NMR relaxation studies of backbone and side chain motion of the catalytic tyrosine residue in free and steroid-bound delta 5-3-ketosteroid isomerase. Biochemistry 35:1525-32
Zhao, Q; Abeygunawardana, C; Talalay, P et al. (1996) NMR evidence for the participation of a low-barrier hydrogen bond in the mechanism of delta 5-3-ketosteroid isomerase. Proc Natl Acad Sci U S A 93:8220-4
Zhao, Q; Mildvan, A S; Talalay, P (1995) Enzymatic and nonenzymatic polarizations of alpha,beta-unsaturated ketosteroids and phenolic steroids. Implications for the roles of hydrogen bonding in the catalytic mechanism of delta 5-3-ketosteroid isomerase. Biochemistry 34:426-34
Zhao, Q; Li, Y K; Mildvan, A S et al. (1995) Ultraviolet spectroscopic evidence for decreased motion of the active site tyrosine residue of delta 5-3-ketosteroid isomerase by steroid binding. Biochemistry 34:6562-72
Austin, J C; Zhao, Q; Jordan, T et al. (1995) Ultraviolet resonance Raman spectroscopy of delta 5-3-ketosteroid isomerase revisited: substrate polarization by active-site residues. Biochemistry 34:4441-7
Wu, P; Li, Y K; Talalay, P et al. (1994) Characterization of the three tyrosine residues of delta 5-3-ketosteroid isomerase by time-resolved fluorescence and circular dichroism. Biochemistry 33:7415-22
Li, Y K; Kuliopulos, A; Mildvan, A S et al. (1993) Environments and mechanistic roles of the tyrosine residues of delta 5-3-ketosteroid isomerase. Biochemistry 32:1816-24
Austin, J C; Kuliopulos, A; Mildvan, A S et al. (1992) Substrate polarization by residues in delta 5-3-ketosteroid isomerase probed by site-directed mutagenesis and UV resonance Raman spectroscopy. Protein Sci 1:259-70

Showing the most recent 10 out of 16 publications