This proposal deals with the synthesis of pregnenolone, the precursor to progesterone and its inhibition in vitro and in vivo. The in vitro studies will involve the purification and characterization of corpus luteum cytochrome P-450. The interactions at the active site will be studied by equilibrium binding, optic and paramagnetic spectral techniques with radioactive and spin-labeled substrate and inhibitor analogs. Other studies will deal with the target effects of the progesterone molecule including its binding energies and those of monosubstituted congeners. Appropriate substances of potential pharmacologic significance will be assessed further by bioassay. Purification of placental aromatase will also be undertaken and characteristics of the purified protein and its substrate and inhibitor sites will be studied.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK010081-21
Application #
3224663
Study Section
Endocrinology Study Section (END)
Project Start
1977-04-01
Project End
1987-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
21
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Harvard University
Department
Type
Schools of Public Health
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115