Abstract

The aims of the research proposed here are to study the structure of the insulin receptor and the mechanism by which insulin activates membrane transport systems, in particular the (NA+, K+) ATPase and other Na+ transporters.
One aim i s to identify functional domains of the insulin receptor involved in monomer-monomer interactions, disulfide bond formation and insulin binding. Another aim is to determine and isolate the components involved in transducing the signal of the insulin receptor interaction to the (Na+, K+) ATPase and other Na+ transporters.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
2R01DK027626-06
Application #
3228400
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1981-03-01
Project End
1991-02-28
Budget Start
1986-03-20
Budget End
1987-02-28
Support Year
6
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Type
Schools of Arts and Sciences
DUNS #
071723621
City
Cambridge
State
MA
Country
United States
Zip Code
02138

  Publications

Wu, James Jianping; Guidotti, Guido (2004) Proreceptor dimerization is required for insulin receptor post-translational processing. J Biol Chem 279:25765-73
Wu, James Jianping; Guidotti, Guido (2002) Construction and characterization of a monomeric insulin receptor. J Biol Chem 277:27809-17
Lee, K; Jung, J; Kim, M et al. (2001) Interaction of the alpha subunit of Na,K-ATPase with cofilin. Biochem J 353:377-85
Chen, W; Guidotti, G (2001) Soluble apyrases release adp during ATP hydrolysis. Biochem Biophys Res Commun 282:90-5
Coppi, M V; Compton, L A; Guidotti, G (1999) Isoform-specific effects of charged residues at borders of the M1-M2 loop of the Na,K-ATPase alpha subunit. Biochemistry 38:2494-505
Coppi, M V; Guidotti, G (1997) The alpha2L111R,N122D isoform of the Na,K-ATPase expressed in HeLa cells does not undergo an adipocyte-like increase in activity in response to insulin. Biochem Biophys Res Commun 236:444-8
Coppi, M V; Guidotti, G (1997) Intracellular localization of Na,K-ATPase alpha2 subunit mutants. Arch Biochem Biophys 346:312-21
Coppi, M V; Guidotti, G (1997) Ubiquitination of Na,K-ATPase alpha1 and alpha2 subunits. FEBS Lett 405:281-4
Santy, L C; Guidotti, G (1997) Expression of a single gene produces both forms of skeletal muscle cyclic nucleotide-gated channels. Am J Physiol 273:E1140-8
Santy, L C; Guidotti, G (1996) Reconstitution and characterization of two forms of cyclic nucleotide-gated channels from skeletal muscle. Am J Physiol 271:E1051-60

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