Abstract

Several series of experiments are planned in continuing work on two neural peptides, substance P and neurotensin, that have been isolated, sequenced, and synthesized in this laboratory. The studies outlined include a) mapping the distribution of these peptides in brain and peripheral nerve. b) delineation of substance P or neurotensin-containing tracts within the central nervous system. c) the release of substance P and neurotensin from neural tissue in vitro and in vivo. d) the chemical characterization of substance P and neurotensin in plasma. e) manipulation of the plasma levels of immunoreactive substance P and neurotensin.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK029876-07
Application #
3229100
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1980-12-01
Project End
1987-05-31
Budget Start
1986-06-01
Budget End
1987-05-31
Support Year
7
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Massachusetts Medical School Worcester
Department
Type
Schools of Medicine
DUNS #
660735098
City
Worcester
State
MA
Country
United States
Zip Code
01655

  Publications

Alexander, M J; Leeman, S E (1998) Widespread expression in adult rat forebrain of mRNA encoding high-affinity neurotensin receptor. J Comp Neurol 402:475-500
Castagliuolo, I; Leeman, S E; Bartolak-Suki, E et al. (1996) A neurotensin antagonist, SR 48692, inhibits colonic responses to immobilization stress in rats. Proc Natl Acad Sci U S A 93:12611-5
Kage, R; Hershey, A D; Krause, J E et al. (1995) Characterization of the substance P (NK-1) receptor in tunicamycin-treated transfected cells using a photoaffinity analogue of substance P. J Neurochem 64:316-21
Alexander, M J; Leeman, S E (1994) Estrogen-inducible neurotensin immunoreactivity in the preoptic area of the female rat. J Comp Neurol 345:496-509
Kage, R; Leeman, S E; Boyd, N D (1993) Biochemical characterization of two different forms of the substance P receptor in rat submaxillary gland. J Neurochem 60:347-51
Alexander, M J (1993) Estrogen-regulated synthesis of neurotensin in neurosecretory cells of the hypothalamic arcuate nucleus in the female rat. Endocrinology 133:1809-16
Alexander, M J; Leeman, S E (1992) Neurotensin gene expression in the rat preoptic area. Implications for the regulation of reproduction. Ann N Y Acad Sci 668:70-89
Jonassen, J A; Mullikin-Kilpatrick, D; Hoogasian, J et al. (1992) Regulation of adenohypophyseal messenger RNAs in female rats by age, hypothyroidism, estradiol and neonatal androgenization. Mol Cell Endocrinol 84:55-64
Shamgochian, M D; Leeman, S E (1992) Substance P stimulates luteinizing hormone secretion from anterior pituitary cells in culture. Endocrinology 131:871-5
Boyd, N D; White, C F; Cerpa, R et al. (1991) Photoaffinity labeling the substance P receptor using a derivative of substance P containing p-benzoylphenylalanine. Biochemistry 30:336-42

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