Abstract

This application is aimed at elucidation of certain conformational and mechanistic aspects of the gonadotropins, human choriogonadotropin and lutropin. The Physicochemical studies will include electron paramagnetic resonance, nuclear magnetic resonance and circular dichroism. The mechanistic studies will be directed mainly toward actions in Leydig tumor cells; these studies include the role of gonadotropin carbohydrate in eliciting a biological response, characterization of gonadotropin induced proteins, actions of estrogen and the action of a putative lutropin variant in kidneys. Physicochemical studies will be conducted on beta-endorphin and derived peptides, and the epsilon opiate receptor, which exhibits beta-endorphin specificity, will be characterized. Lastly, the regulation of pro-opiomelanocortin will be studied in transformed Leydig cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK033973-04
Application #
3232389
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1983-09-01
Project End
1990-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
4
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Miami School of Medicine
Department
Type
Schools of Medicine
DUNS #
City
Miami
State
FL
Country
United States
Zip Code
33101

  Publications

Chen, Tsuey-Ming; Czerwiec, Frank S; Puett, David (2016) Steroidogenesis and early response gene expression in MA-10 Leydig tumor cells following heterologous receptor down-regulation and cellular desensitization. Biochem Biophys Rep 5:305-312
Cui, Juan; Yin, Yanbin; Ma, Qin et al. (2015) Comprehensive characterization of the genomic alterations in human gastric cancer. Int J Cancer 137:86-95
Bowman, Paula B; Puett, David (2014) Electron paramagnetic resonance spectroscopy of nitroxide-labeled calmodulin. Protein J 33:267-77
Cui, Juan; Xu, Ying; Puett, David (2013) Microarray-based transcriptome profiling of ovarian cancer cells. Methods Mol Biol 1049:119-37
Cui, Juan; Miner, Brooke M; Eldredge, Joanna B et al. (2011) Regulation of gene expression in ovarian cancer cells by luteinizing hormone receptor expression and activation. BMC Cancer 11:280
Angelova, Krassimira; Felline, Angelo; Lee, Moon et al. (2011) Conserved amino acids participate in the structure networks deputed to intramolecular communication in the lutropin receptor. Cell Mol Life Sci 68:1227-39
DeMars, Geneva; Fanelli, Francesca; Puett, David (2011) The extreme C-terminal region of G?s differentially couples to the luteinizing hormone and beta2-adrenergic receptors. Mol Endocrinol 25:1416-30
Cui, Juan; Eldredge, Joanna B; Xu, Ying et al. (2011) MicroRNA expression and regulation in human ovarian carcinoma cells by luteinizing hormone. PLoS One 6:e21730
Puett, David; Angelova, Krassimira; da Costa, Marcelo Rocha et al. (2010) The luteinizing hormone receptor: insights into structure-function relationships and hormone-receptor-mediated changes in gene expression in ovarian cancer cells. Mol Cell Endocrinol 329:47-55
Puett, David; Angelova, Krassimira (2009) Determining the affinity of hormone-receptor interaction. Methods Mol Biol 590:1-20

Showing the most recent 10 out of 83 publications