Anti-neutrophil cytoplasmic autoantibodies (ANCA) are sensitive and specific serological markers of systemic necrotizing vasculitis, including Wegener's granulomatosis, microscopic polyangitis, Churg-Strauss syndrome and renal limited necrotizing glomerulonephritis. In vitro and in vivo evidence suggest that ANCA may participate in the pathogenic process. The long-term objectives of this proposal are to extend the knowledge of the clinical, pathologic, immunological and genetic characteristics of ANCA associated disease, and to elucidate the basic immunobiology, immunogenics, and pathogenesis of the ANCA autoimmune response and ANCA-associated diseases. The goal of Aim 1 is to elucidate the clinical, pathologic, serologic, and genetic characteristics of ANCA-associated diseases in a cohort of over 250 patients. It is hypothesized that: 1) ANCA are useful in monitoring disease activity, including patients with end-stage renal disease; 2) ANCA correlated with the pathologic activity; and 3) the ANCA autoimmune response is associated with restricted MHC class II genotype.
The second Aim will investigate the origin, evolution, epitope specificity and immunogenics of the ANCA immune response. It is hypothesized that: 1) the ANCA autoimmune response is directed against a limited number of immunodominant epitopes; 2) ANCA production results from the expression of limited combinations of VDJ genes; 3) precursor B cells clones that react with DNA and MPO can be selectively expanded by antigen stimulation to produce either anti-MPO or anti-DNA autoantibodies; and 4) transgenic mice with anti-potentially pathogenic anti-MPO antibodies will sustain tolerance by down regulating the transgenic clone or will develop potentially pathogenic anti-MPO antibodies. .
The final aim will explore the pathogenesis of ANCA-associated vasculitis, based on the hypothesis that ANCA are pathogenic, using an autoimmune strain of mice SCG/Kg and humanized SCID mice. It is postulated that: 1) in the vasculitic environment, ANCA cytokines and chemokines modify neutrophil apoptosis thus altering the balance of neutrophil clearance and neutrophil-mediated injury; 2) the ANCA antigens proteinase 3 and elastase, induce endothelial cell apoptosis; and 3) neutrophil and endothelial apoptosis are involved in the pathogenesis of vasculitis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK040208-12
Application #
6137986
Study Section
Pathology A Study Section (PTHA)
Program Officer
Hirschman, Gladys H
Project Start
1989-01-01
Project End
2000-12-31
Budget Start
2000-01-01
Budget End
2000-12-31
Support Year
12
Fiscal Year
2000
Total Cost
$231,463
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Yang, J J; Preston, G A; Pendergraft, W F et al. (2001) Internalization of proteinase 3 is concomitant with endothelial cell apoptosis and internalization of myeloperoxidase with generation of intracellular oxidants. Am J Pathol 158:581-92
Jethwa, H S; Clarke, S H; Itoh-Lindstrom, Y et al. (2000) Restriction in V kappa gene use and antigen selection in anti-myeloperoxidase response in mice. J Immunol 165:3890-7
Kettritz, R; Gaido, M L; Haller, H et al. (1998) Interleukin-8 delays spontaneous and tumor necrosis factor-alpha-mediated apoptosis of human neutrophils. Kidney Int 53:84-91
Kettritz, R; Jennette, J C; Falk, R J (1997) Crosslinking of ANCA-antigens stimulates superoxide release by human neutrophils. J Am Soc Nephrol 8:386-94
Kettritz, R; Falk, R J; Jennette, J C et al. (1997) Neutrophil superoxide release is required for spontaneous and FMLP-mediated but not for TNF alpha-mediated apoptosis. J Am Soc Nephrol 8:1091-100
Nachman, P H; Reisner, H M; Yang, J J et al. (1996) Shared idiotypy among patients with myeloperoxidase-anti-neutrophil cytoplasmic autoantibody associated glomerulonephritis and vasculitis. Lab Invest 74:519-27
Yang, J J; Tuttle, R; Falk, R J et al. (1996) Frequency of anti-bactericidal/permeability-increasing protein (BPI) and anti-azurocidin in patients with renal disease. Clin Exp Immunol 105:125-31
Hogan, S L; Nachman, P H; Wilkman, A S et al. (1996) Prognostic markers in patients with antineutrophil cytoplasmic autoantibody-associated microscopic polyangiitis and glomerulonephritis. J Am Soc Nephrol 7:23-32
Yang, J J; Kettritz, R; Falk, R J et al. (1996) Apoptosis of endothelial cells induced by the neutrophil serine proteases proteinase 3 and elastase. Am J Pathol 149:1617-26
Nachman, P H; Hogan, S L; Jennette, J C et al. (1996) Treatment response and relapse in antineutrophil cytoplasmic autoantibody-associated microscopic polyangiitis and glomerulonephritis. J Am Soc Nephrol 7:33-9

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