In the last decade, major scientific advances in bioimaging based on the use of ultrashort (picosecond- and femtosecond-duration) optical pulses have occurred, and initial demonstrations of short-pulse diagnosis and treatment of disease have also been reported. Examples include multiphoton microscopy, optical coherence tomography imaging, and femtosecond corneal surgery. A relative weakness of optical imaging techniques is their poor depth-penetration: imaging is limited to thin (<1 mm) samples and superficial tissue. Images of structures much deeper in tissue could be obtained with wavelengths beyond 1 micron; the 1-1.3micron range appears to offer order-of-magnitude advantages. Femtosecond-pulse sources that are tunable across this wavelength range will be developed. The sources will be designed for the needs of nonlinear microscopies and optical coherence tomography. The heart of the sources will be femtosecond fiber lasers, which offer practical advantages over existing solid-state lasers including greater stability and lower cost. The developed lasers will be used in two separate collaborative studies. At Cornell University, third- harmonic generation microscopy will be applied to in vivo characterization of morphological changes in cells caused by optically-induced small-scale strokes in the rat cortex. The images will allow us to map the effects of the vascular lesion on unlabeled neural cells. At the University of California at Irvine, second-harmonic optical coherence tomography will be employed to monitor the progression of cancer in the hamster cheek pouch model. The development of new and enhanced capabilities for visualizing tissue will aid in the diagnosis and treatment of disease. In particular, third-harmonic microscopy will provide detailed morphological information about tissue that is accessible directly or by endoscopy, without exogenous labels. Second-harmonic optical coherence tomography has potential for early detection of oral cancers.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
3R01EB002019-10A2S1
Application #
7547720
Study Section
Biomedical Imaging Technology Study Section (BMIT)
Program Officer
Zhang, Yantian
Project Start
1995-08-01
Project End
2010-05-31
Budget Start
2008-01-01
Budget End
2008-05-31
Support Year
10
Fiscal Year
2008
Total Cost
$23,751
Indirect Cost
Name
Cornell University
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
872612445
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Fu, Walter; Wright, Logan G; Wise, Frank W (2017) High-power femtosecond pulses without a modelocked laser. Optica 4:831-834
Liu, Zhanwei; Ziegler, Zachary M; Wright, Logan G et al. (2017) Megawatt peak power from a Mamyshev oscillator. Optica 4:649-654
Fu, Walter; Tang, Yuxing; McComb, Timothy S et al. (2017) Limits of Femtosecond Fiber Amplification by Parabolic Pre-Shaping. J Opt Soc Am B 34:A37-A42
Farrar, Matthew J; Rubin, Jonathan D; Diago, Darcy M et al. (2015) Characterization of blood flow in the mouse dorsal spinal venous system before and after dorsal spinal vein occlusion. J Cereb Blood Flow Metab 35:667-75
Chong, Andy; Wright, Logan G; Wise, Frank W (2015) Ultrafast fiber lasers based on self-similar pulse evolution: a review of current progress. Rep Prog Phys 78:113901
Lamb, Erin S; Wise, Frank W (2015) Multimodal fiber source for nonlinear microscopy based on a dissipative soliton laser. Biomed Opt Express 6:3248-55
Liu, Hui; Liu, Zhanwei; Lamb, Erin S et al. (2014) Self-similar erbium-doped fiber laser with large normal dispersion. Opt Lett 39:1019-21
Lamb, Erin S; Wright, Logan G; Wise, Frank W (2014) Divided-pulse lasers. Opt Lett 39:2775-7
Farrar, Matthew J; Schaffer, Chris B (2014) A procedure for implanting a spinal chamber for longitudinal in vivo imaging of the mouse spinal cord. J Vis Exp :
Cianchetti, Flor A; Kim, Dong Hwan; Dimiduk, Sally et al. (2013) Stimulus-evoked calcium transients in somatosensory cortex are temporarily inhibited by a nearby microhemorrhage. PLoS One 8:e65663

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