The objectives of this study are to understand the organization of synaptic contacts in the retina and the biochemical mechanisms underlying synaptic neurotransmission. Specific projects presently ongoing or planned for the next five years include: 1) An electron microscopic analysis of the synaptic organization of amino acid-accumulating and peptide-containing amacrine cells in the rabbit retina. 2) A biochemical study of dopamine-dependent adenylate cyclase activity and dopamine-dependent protein phosphorylation in isolated carp horizontal cells. 3) A biochemical study of vasoactive intestinal peptide (VIP) stimulation of cyclic AMP accumulation in the carp retina. 4) A combined anatomical and biochemical study of carp horizontal cells maintained in cell culture. 5) A biochemical study of the properties of the dopamine receptors on isolated carp horizontal cells and the antigenic properties of isolated carp horizontal cells.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY000811-14
Application #
3255550
Study Section
(VID)
Project Start
1976-12-01
Project End
1986-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Harvard University
Department
Type
Schools of Arts and Sciences
DUNS #
071723621
City
Cambridge
State
MA
Country
United States
Zip Code
Wasfy, Meagan M; Matsui, Jonathan I; Miller, Jessica et al. (2014) myosin 7aa(-/-) mutant zebrafish show mild photoreceptor degeneration and reduced electroretinographic responses. Exp Eye Res 122:65-76
Moyano, Miguel; Porteros, Angel; Dowling, John E (2013) The effects of nicotine on cone and rod b-wave responses in larval zebrafish. Vis Neurosci 30:141-5
Li, Yong N; Tsujimura, Taro; Kawamura, Shoji et al. (2012) Bipolar cell-photoreceptor connectivity in the zebrafish (Danio rerio) retina. J Comp Neurol 520:3786-802
Le, Hong-Gam T; Dowling, John E; Cameron, D Joshua (2012) Early retinoic acid deprivation in developing zebrafish results in microphthalmia. Vis Neurosci 29:219-28
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Emran, Farida; Rihel, Jason; Adolph, Alan R et al. (2010) Zebrafish larvae lose vision at night. Proc Natl Acad Sci U S A 107:6034-9
Li, Yong N; Matsui, Jonathan I; Dowling, John E (2009) Specificity of the horizontal cell-photoreceptor connections in the zebrafish (Danio rerio) retina. J Comp Neurol 516:442-53
Emran, Farida; Rihel, Jason; Dowling, John E (2008) A behavioral assay to measure responsiveness of zebrafish to changes in light intensities. J Vis Exp :
Kojima, Daisuke; Torii, Masaki; Fukada, Yoshitaka et al. (2008) Differential expression of duplicated VAL-opsin genes in the developing zebrafish. J Neurochem 104:1364-71

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