Abstract

The overall goal of this study is to determine the nature and the regulation of the cytoskeleton-membrane interactions in bovine and human lens fiber cells. Such knowledge may enhance our understanding of fiber morphogenesis, aging, and ultimately the cataractous process. Specifically we plan to analyse the interactions of protein 4-1, fordrin and actin with each other, and determine the intrinsic membrane protein to which this membrane cytoskeleton is linked. The role of Ca2+, calmodulin, phosphorylations, and phosphatidylinositol on the integrity of the cytoskeleton and its interactions will be explored. The interactions of beaded-chain filaments, and vinculin with the membrane will be determined, as well as the specific cytoskeletal linkage at unit and gap junction regions of the fiber cell plasma membrane. The changing cytoskeletal pattern of the human lens will be studied in relation to aging and cataractogenesis and the role fo Ca2+-activated protease in cytoskeletal degradation will be determined.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY001417-15
Application #
3255937
Study Section
(SSS)
Project Start
1977-09-01
Project End
1990-08-31
Budget Start
1988-09-01
Budget End
1989-08-31
Support Year
15
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Wayne State University
Department
Type
Schools of Medicine
DUNS #
City
Detroit
State
MI
Country
United States
Zip Code
48202

  Publications

Bagchi, M; Ansari, S A; Katar, M et al. (1997) Nonchromatin nuclear proteins of mammalian lens epithelial cells. J Cell Biochem 64:644-50
Yao, R; Alcala, J; Maisel, H (1996) Developmental changes in glycoconjugate composition during chick lens morphogenesis. Exp Eye Res 62:419-31
Yao, R; Crossland, W; Maisel, H (1996) Electron microscopic detection of glycoconjugates in the chicken lens. Exp Eye Res 63:705-11
Sawada, K; Agata, J; Eguchi, G et al. (1995) The predicted structure of chick lens CP49 and a variant thereof, CP49ins, the first vertebrate cytoplasmic intermediate filament protein with a lamin-like insertion in helix 1B. Curr Eye Res 14:545-53
Bagchi, M; Van Wijnen, A; Katar, M et al. (1995) Sequence-specific DNA binding activities of nuclear matrix proteins of mammalian lens epithelial cells. J Cell Biochem 58:1-5
Katar, M; Alcala, J; Maisel, H (1993) NCAM of the mammalian lens. Curr Eye Res 12:191-6
Orii, H; Agata, K; Sawada, K et al. (1993) Evidence that the chick lens cytoskeletal protein CP 49 belongs to the family of intermediate filament proteins. Curr Eye Res 12:583-8
Watanabe, M; Kobayashi, H; Yao, R et al. (1992) Adhesion and junction molecules in embryonic and adult lens cell differentiation. Acta Ophthalmol Suppl :46-52
Lieska, N; Yang, H Y; Maisel, H (1991) Reconstitution of the filamentous backbone of lens beaded-chain filaments from a purified 49kD polypeptide. Curr Eye Res 10:1037-48
Maisel, H; Atreya, P (1990) N-cadherin detected in the membrane fraction of lens fiber cells. Experientia 46:222-3

Showing the most recent 10 out of 23 publications