Abstract

A study of the natural history, pathogenesis, and treatment of experimental retinal detachment caused by retinal laceration complicated by intravitreous blood is proposed. The study will test a hypothetical model which involves and interplay of posterior vitreous detachment, pure traction retinal detachment, and rhegmatogenous retinal detachment. A reproducible experimental primate model of retinal laceration has been developed for these investigations. A rabbit model of posterior vitreous detachment will also be used in studies of vitreoretinal lesion, for reasons of econony. The models will be studied at various times after injury with ultrasonography and electroretinography. Extent of initial membrane formation and detachment will be carefully described by dissection microscopy and light and electron microscopy (TEM and SEM). Additional studies of vitreoretinal adhesion in rabbits and monkeys will identify the distribution of fibronectin and laminin at the vitreoretinal interface. Finally, the efficacy of vitrectomy, buckling procedures, and cryosurgery in controlling the adverse effects of retinal laceration will be evaluated in the model. These studies represent a logical extension of our past work on penetrating ocular injuries of the pars plana and corneoscleral junction, and may provide a rational basis for improved therapy of often catastrophic ocular injuries.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY002061-10
Application #
3256469
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1978-02-01
Project End
1988-02-29
Budget Start
1987-03-01
Budget End
1988-02-29
Support Year
10
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Southern California
Department
Type
Schools of Medicine
DUNS #
041544081
City
Los Angeles
State
CA
Country
United States
Zip Code
90033

  Publications

Zhu, DanHong; Sreekumar, Parameswaran G; Hinton, David R et al. (2010) Expression and regulation of enzymes in the ceramide metabolic pathway in human retinal pigment epithelial cells and their relevance to retinal degeneration. Vision Res 50:643-51
He, Shikun; Kumar, S Ram; Zhou, Peng et al. (2010) Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro. Invest Ophthalmol Vis Sci 51:543-52
Sreekumar, Parameswaran G; Ding, Yi; Ryan, Stephen J et al. (2009) Regulation of thioredoxin by ceramide in retinal pigment epithelial cells. Exp Eye Res 88:410-7
He, Shikun; Chen, Youxin; Khankan, Rima et al. (2008) Connective tissue growth factor as a mediator of intraocular fibrosis. Invest Ophthalmol Vis Sci 49:4078-88
Yaung, Jennifer; Kannan, Ram; Wawrousek, Eric F et al. (2008) Exacerbation of retinal degeneration in the absence of alpha crystallins in an in vivo model of chemically induced hypoxia. Exp Eye Res 86:355-65
Yaung, Jennifer; Jin, Manlin; Barron, Ernesto et al. (2007) alpha-Crystallin distribution in retinal pigment epithelium and effect of gene knockouts on sensitivity to oxidative stress. Mol Vis 13:566-77
Gamulescu, Maria-Andreea; Chen, Youxin; He, Shikun et al. (2006) Transforming growth factor beta2-induced myofibroblastic differentiation of human retinal pigment epithelial cells: regulation by extracellular matrix proteins and hepatocyte growth factor. Exp Eye Res 83:212-22
Sreekumar, Parameswaran G; Kannan, Ram; Yaung, Jennifer et al. (2005) Protection from oxidative stress by methionine sulfoxide reductases in RPE cells. Biochem Biophys Res Commun 334:245-53
Jin, Manlin; Yaung, Jennifer; Kannan, Ram et al. (2005) Hepatocyte growth factor protects RPE cells from apoptosis induced by glutathione depletion. Invest Ophthalmol Vis Sci 46:4311-9
Hoffmann, Stephan; He, Shikun; Jin, Manlin et al. (2005) A selective cyclic integrin antagonist blocks the integrin receptors alphavbeta3 and alphavbeta5 and inhibits retinal pigment epithelium cell attachment, migration and invasion. BMC Ophthalmol 5:16

Showing the most recent 10 out of 61 publications