Three sets of experiments are proposed to continue our research on the prenatal development of the mammalian visual system. In the next 5 years we will focus on the normal ontogeny of retinal ganglion cells and their axonal projection patterns. First, we will study the ontogeny of regional specialization within the ganglion cell layer. In these experiments retinal ganglion cells will be identified unequivocally by labeling these neurons following injections of the retrograde tracer horseradish peroxidase into the optic chiasm of fetal animals at known gestational ages. As a result it will be possible to study the spatial and temporal patterns of retinal ganglion cell outgrowth as well as the morphological differentiation of these neurons. This information is essential in order to increase our understanding of the processes underlying the maturation of the retinal ganglion cell layer. In the second set of experiments, the formation of retinal nasotemporal decussation patterns will be studied by injecting different fluorescent tracers (diamidino yellow and rhodamine-labeled microspheres) into the main retinorecipient regions on each side of the brain. This will permit an accurate assessment of the degree of nasotemporal overlap in the distribution of ganglion cells whose axons project to the same or opposite side of the brain. It will also be feasible to determine the presence of bilaterally projecting ganglion cells. In the third set of studies, the topographical organization of developing crossed and uncrossed retinofugal projections will be examined using two complementary anatomical methods. These studies will permit us to assess the degree to which developmental refinements and corrections may contribute to the topographical organization of retinal projections. The immediate significance of this research program is to increase our understanding of the events underlying the normal development of the visual system. These results will also provide new knowledge of general principles governing the development of the mammalian brain.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY003991-07
Application #
3258471
Study Section
Visual Sciences B Study Section (VISB)
Project Start
1982-08-01
Project End
1991-03-31
Budget Start
1989-04-01
Budget End
1990-03-31
Support Year
7
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of California Davis
Department
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618
Failor, Samuel; Chapman, Barbara; Cheng, Hwai-Jong (2015) Retinal waves regulate afferent terminal targeting in the early visual pathway. Proc Natl Acad Sci U S A 112:E2957-66
Speer, Colenso M; Sun, Chao; Liets, Lauren C et al. (2014) Eye-specific retinogeniculate segregation proceeds normally following disruption of patterned spontaneous retinal activity. Neural Dev 9:25
Dursun, Ilknur; Jakubowska-Dogru, Ewa; Elibol-Can, Birsen et al. (2013) Effects of early postnatal alcohol exposure on the developing retinogeniculate projections in C57BL/6 mice. Alcohol 47:173-9
Dursun, Ilknur; Jakubowska-Dogru, Ewa; van der List, Deborah et al. (2011) Effects of early postnatal exposure to ethanol on retinal ganglion cell morphology and numbers of neurons in the dorsolateral geniculate in mice. Alcohol Clin Exp Res 35:2063-74
Rubin, Carol M; van der List, Deborah A; Ballesteros, Jose M et al. (2011) Mouse mutants for the nicotinic acetylcholine receptor ß2 subunit display changes in cell adhesion and neurodegeneration response genes. PLoS One 6:e18626
Speer, Colenso M; Sun, Chao; Chapman, Barbara (2011) Activity-dependent disruption of intersublaminar spaces and ABAKAN expression does not impact functional on and off organization in the ferret retinogeniculate system. Neural Dev 6:7
Murray, Karl D; Rubin, Carol M; Jones, Edward G et al. (2008) Molecular correlates of laminar differences in the macaque dorsal lateral geniculate nucleus. J Neurosci 28:12010-22
Gunhan, Emine; Swe, Mimi; Palazoglu, Mine et al. (2008) Expression and purification of cysteine introduced recombinant saporin. Protein Expr Purif 58:203-9
Sun, Chao; Speer, Colenso M; Wang, Guo-Yong et al. (2008) Epibatidine application in vitro blocks retinal waves without silencing all retinal ganglion cell action potentials in developing retina of the mouse and ferret. J Neurophysiol 100:3253-63
Sun, Chao; Warland, David K; Ballesteros, Jose M et al. (2008) Retinal waves in mice lacking the beta2 subunit of the nicotinic acetylcholine receptor. Proc Natl Acad Sci U S A 105:13638-43

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