Abstract

The overall objective of the Proposed research program is to throw more light on the molecular mechanisms underlying prostaglandin (PG) release, and to elucidate the relationship between adrenergic receptor activation and PG formation in ocular tissues. To achieve these objectives we propose to carry out the following experiments: (1) Investigate the release of basal (endogenous) arachidonic acid from phospholipids of rabbit and bovine irides and its subsequent conversion into PGE2. Methodology for these studies will be developed. (2) Investigate the effects of adrenergic drugs, Ca++, Ca++-inonophore and phospholipase A2 inhibitors on the basal release of arachidonate and PGE2 by rabbit and bovine irides. (3) Investigate the effects of in vivo electrical stimulation of the sympathetic nerve on the release of arachidonate and PGE2 by the rabbit iris. (4) Determine if any of the agents which were found to affect arachidonate and PGE2 release from ocular tissues can be used as anti-inflammatory drugs in the eye.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY004387-08
Application #
3258781
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1981-12-01
Project End
1992-03-31
Budget Start
1989-04-01
Budget End
1990-03-31
Support Year
8
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Medical College of Georgia (MCG)
Department
Type
Schools of Medicine
DUNS #
City
Augusta
State
GA
Country
United States
Zip Code
30912

  Publications

Kaddour-Djebbar, I; Ansari, H R; Akhtar, R A et al. (2005) Species differences in the effects of prostanoids on MAP kinase phosphorylation, myosin light chain phosphorylation and contraction in bovine and cat iris sphincter smooth muscle. Prostaglandins Leukot Essent Fatty Acids 72:49-57
Ansari, Habib R; Kaddour-Djebbar, Ismail; Abdel-Latif, Ata A (2004) Involvement of Ca2+ channels in endothelin-1-induced MAP kinase phosphorylation, myosin light chain phosphorylation and contraction in rabbit iris sphincter smooth muscle. Cell Signal 16:609-19
Ansari, Habib R; Kaddour-Djebbar, Ismail; Abdel-Latif, Ata A (2004) Effects of prostaglandin F2alpha, latanoprost and carbachol on phosphoinositide turnover, MAP kinases, myosin light chain phosphorylation and contraction and functional existence and expression of FP receptors in bovine iris sphincter. Exp Eye Res 78:285-96
Sharif, Naj A; Crider, Julie Y; Husain, Shahid et al. (2003) Human ciliary muscle cell responses to FP-class prostaglandin analogs: phosphoinositide hydrolysis, intracellular Ca2+ mobilization and MAP kinase activation. J Ocul Pharmacol Ther 19:437-55
Ansari, Habib R; Davis, Angela M; Kaddour-Djebbar, Ismail et al. (2003) Effects of prostaglandin F2alpha and latanoprost on phosphoinositide turnover, myosin light chain phosphorylation and contraction in cat iris sphincter. J Ocul Pharmacol Ther 19:217-31
Husain, Shahid; Kaddour-Djebbar, Ismail; Abdel-Latif, Ata A (2002) Alterations in arachidonic acid release and phospholipase C-beta(1) expression in glaucomatous human ciliary muscle cells. Invest Ophthalmol Vis Sci 43:1127-34
Ansari, H R; Husain, S; Abdel-Latif, A A (2001) Activation of p42/p44 mitogen-activated protein kinase and contraction by prostaglandin F2alpha, ionomycin, and thapsigargin in cat iris sphincter smooth muscle: inhibition by PD98059, KN-93, and isoproterenol. J Pharmacol Exp Ther 299:178-86
Abdel-Latif, A A (2001) Cross talk between cyclic nucleotides and polyphosphoinositide hydrolysis, protein kinases, and contraction in smooth muscle. Exp Biol Med (Maywood) 226:153-63
Husain, S; Abdel-Latif, A A (2001) Effects of prostaglandin F(2alpha)and carbachol on MAP kinases, cytosolic phospholipase A(2)and arachidonic acid release in cat iris sphincter smooth muscle cells. Exp Eye Res 72:581-90
Ali, N; Yousufzai, S Y; Abdel-Latif, A A (2000) Activation of particulate guanylate cyclase by adrenomedullin in cultured SV-40 transformed cat iris sphincter smooth muscle (SV-CISM-2) cells. Cell Signal 12:491-8

Showing the most recent 10 out of 76 publications