Abstract

Dynamic studies of the initial events of photoreception and transduction in the primary visual process are proposed. Kinetic spectroscopy enables us to investigate the sequential intermediates which are initiated by the absorption of a photon by the molecular photoreceptor rhodopsin. Experiments are designed to elucidate the nature of the conformational intermediates, their potential energy surfaces, and their kinetic transformations. The link between these intermediate events, and the next step in triggering a change in the membrane potential of the photoreceptor cell, is sought. The mechanism of activation of photoreceptor cGMP phosphodiesterase by rhodopsin is being examined to determine whether the activation is part of the cellular triggering and/or a response modulating cellular sensitivity.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY004801-04
Application #
3259332
Study Section
Biophysics and Biophysical Chemistry A Study Section (BBCA)
Project Start
1982-09-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Purdue University
Department
Type
Schools of Arts and Sciences
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907

  Publications

Applebury, M L; Farhangfar, F; Glosmann, M et al. (2007) Transient expression of thyroid hormone nuclear receptor TRbeta2 sets S opsin patterning during cone photoreceptor genesis. Dev Dyn 236:1203-12
Fasick, Jeffry I; Applebury, Meredithe L; Oprian, Daniel D (2002) Spectral tuning in the mammalian short-wavelength sensitive cone pigments. Biochemistry 41:6860-5
Applebury, M L; Antoch, M P; Baxter, L C et al. (2000) The murine cone photoreceptor: a single cone type expresses both S and M opsins with retinal spatial patterning. Neuron 27:513-23
Wilson, C J; Copeland, R A (1997) Spectroscopic characterization of arrestin interactions with competitive ligands: study of heparin and phytic acid binding. J Protein Chem 16:755-63
Wagner, E; McCaffery, P; Mey, J et al. (1997) Retinoic aid increases arrestin mRNA levels in the mouse retina. FASEB J 11:271-5
Aparicio, J G; Applebury, M L (1995) The bovine photoreceptor outer segment guanylate cyclase: purification, kinetic properties, and molecular size. Protein Expr Purif 6:501-11
Wood, R L; Park, K H; Gierow, J P et al. (1994) Immunogold localization of prolactin in acinar cells of lacrimal gland. Adv Exp Med Biol 350:75-7
Pierce, M E; Sheshberadaran, H; Zhang, Z et al. (1993) Circadian regulation of iodopsin gene expression in embryonic photoreceptors in retinal cell culture. Neuron 10:579-84
Lem, J; Applebury, M L; Falk, J D et al. (1991) Tissue-specific and developmental regulation of rod opsin chimeric genes in transgenic mice. Neuron 6:201-10
Leser, G P; Nicoll, D A; Applebury, M L (1991) Distinctive properties of the purified Na-Ca exchanger from rod outer segments. Ann N Y Acad Sci 639:222-33

Showing the most recent 10 out of 21 publications