Pre-oculomotor neurons in the superior colliculus, reticular formation, medial longitudional fasiculus, brachium conjunctivum, and Y-group of the vestibular nuclei will be studied with intracellular and intraaxonal recording techniques in anesthetized and alert squirrel monkeys. In anesthetized animals selected neuronal somata, electrophysiologically identified by anti- and orthodromic stimulation will be injected with horseradish peroxidase (HRP) for subsequent histological study and reconstruction at the light microscopic level. These same and other neurons will be studied in alert, trained, behaving animals. For alert animal studies eye movements will be measured using the magnetic search coil technique and natural and electrial stimulation of the vestibular system will be employed. Intraaxonal recordings will be taken to correlate neuronal firing patterns with eye movements and vestibular stimulation. (Computer aided analysis will be used on- and off-line). After physiological identification selected axons will be injected with HRP for subsequent reconstruction and characterization of axonal trajectories, patterns of collateralization and terminal fields at the light microscopic level. We thus hope to continue to provide structure-function correlation for the pre-oculomotor and oculomotor neurons so well studied at the extracellular level by others in this field. Our data, obtained from real neurons, will serve to evaluate the validity of existing and new models and concepts of oculomotor function.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY005433-08
Application #
3260493
Study Section
Visual Sciences B Study Section (VISB)
Project Start
1983-11-01
Project End
1992-03-31
Budget Start
1990-08-01
Budget End
1992-03-31
Support Year
8
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Heine, Shane A; Highstein, Stephen M; Blazquez, Pablo M (2010) Golgi cells operate as state-specific temporal filters at the input stage of the cerebellar cortex. J Neurosci 30:17004-14
Inagaki, Keiichiro; Heiney, Shane A; Blazquez, Pablo M (2009) Method for the construction and use of carbon fiber multibarrel electrodes for deep brain recordings in the alert animal. J Neurosci Methods 178:255-62