Abstract

This competing renewal consists of 5 specific aims. The first three aims will examine using postembedding immuno-EM combined with cytochrome oxidase histochemistry to examine the neurochemical composition of synapses and neurons in the cytochrome oxidase rich puff and cytochrome oxidase poor regions of layer 2/3 in monkey visual cortex. These same analyses will be done following monocular deprivation to assess how activity might differentially regulate the neurochemical nature of synaptic connections in puff and interpuff regions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY005439-11
Application #
2444280
Study Section
Visual Sciences B Study Section (VISB)
Project Start
1984-07-01
Project End
2000-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
11
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Medical College of Wisconsin
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
073134603
City
Milwaukee
State
WI
Country
United States
Zip Code
53226

  Publications

Dhar, Shilpa S; Ongwijitwat, Sakkapol; Wong-Riley, Margaret T T (2008) Nuclear respiratory factor 1 regulates all ten nuclear-encoded subunits of cytochrome c oxidase in neurons. J Biol Chem 283:3120-9
Ongwijitwat, Sakkapol; Liang, Huan Ling; Graboyes, Evan M et al. (2006) Nuclear respiratory factor 2 senses changing cellular energy demands and its silencing down-regulates cytochrome oxidase and other target gene mRNAs. Gene 374:39-49
Liang, H L; Ongwijitwat, S; Wong-Riley, M T T (2006) Bigenomic functional regulation of all 13 cytochrome c oxidase subunit transcripts in rat neurons in vitro and in vivo. Neuroscience 140:177-90
Liang, Huan Ling; Wong-Riley, Margaret T T (2006) Activity-dependent regulation of nuclear respiratory factor-1, nuclear respiratory factor-2, and peroxisome proliferator-activated receptor gamma coactivator-1 in neurons. Neuroreport 17:401-5
Yang, S J; Liang, H L; Wong-Riley, M T T (2006) Activity-dependent transcriptional regulation of nuclear respiratory factor-1 in cultured rat visual cortical neurons. Neuroscience 141:1181-92
Wong-Riley, Margaret T T; Yang, Shou Jing; Liang, Huan Ling et al. (2005) Quantitative immuno-electron microscopic analysis of nuclear respiratory factor 2 alpha and beta subunits: Normal distribution and activity-dependent regulation in mammalian visual cortex. Vis Neurosci 22:1-18
Ongwijitwat, Sakkapol; Wong-Riley, Margaret T T (2005) Is nuclear respiratory factor 2 a master transcriptional coordinator for all ten nuclear-encoded cytochrome c oxidase subunits in neurons? Gene 360:65-77
Ongwijitwat, Sakkapol; Wong-Riley, Margaret T T (2004) Functional analysis of the rat cytochrome c oxidase subunit 6A1 promoter in primary neurons. Gene 337:163-71
Bai, Xuetao; Wong-Riley, Margaret T T (2003) Neuronal activity regulates protein and gene expressions of GluR2 in postnatal rat visual cortical neurons in culture. J Neurocytol 32:71-8
Wong-Riley, Margaret T T; Jacobs, Paulette (2002) AMPA glutamate receptor subunit 2 in normal and visually deprived macaque visual cortex. Vis Neurosci 19:563-73

Showing the most recent 10 out of 33 publications