Abstract

The investigator has been studying the mechanisms that control retinal development, and now proposes to study the cell cycle regulatory proteins to define their role in this process. The two basic aims of the research program are to determine: (1) whether the differences in proliferation behavior of progenitor cells at different times in retinal development are due to intrinsic or extrinsic differences; and (2) whether any of the known cell cycle genes contribute to the cycling behavior of retinal progenitor cells and the types of progeny they produce. The investigator proposes to use a combination of in vitro and in vivo approaches to address these questions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY008064-13
Application #
6384602
Study Section
Visual Sciences C Study Section (VISC)
Program Officer
Hunter, Chyren
Project Start
1989-04-01
Project End
2003-03-31
Budget Start
2001-04-01
Budget End
2002-03-31
Support Year
13
Fiscal Year
2001
Total Cost
$161,654
Indirect Cost

  Institution

Name
Harvard University
Department
Genetics
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Hafler, Brian P; Surzenko, Natalia; Beier, Kevin T et al. (2012) Transcription factor Olig2 defines subpopulations of retinal progenitor cells biased toward specific cell fates. Proc Natl Acad Sci U S A 109:7882-7
Cherry, Timothy J; Wang, Sui; Bormuth, Ingo et al. (2011) NeuroD factors regulate cell fate and neurite stratification in the developing retina. J Neurosci 31:7365-79
Beier, Kevin T; Samson, Maria Elena S; Matsuda, Takahiko et al. (2011) Conditional expression of the TVA receptor allows clonal analysis of descendents from Cre-expressing progenitor cells. Dev Biol 353:309-20
Bienvenu, Frédéric; Jirawatnotai, Siwanon; Elias, Joshua E et al. (2010) Transcriptional role of cyclin D1 in development revealed by a genetic-proteomic screen. Nature 463:374-8
Jadhav, Ashutosh P; Roesch, Karin; Cepko, Constance L (2009) Development and neurogenic potential of Muller glial cells in the vertebrate retina. Prog Retin Eye Res 28:249-62
Trimarchi, Jeffrey M; Stadler, Michael B; Cepko, Constance L (2008) Individual retinal progenitor cells display extensive heterogeneity of gene expression. PLoS One 3:e1588
Roesch, Karin; Jadhav, Ashutosh P; Trimarchi, Jeffrey M et al. (2008) The transcriptome of retinal Muller glial cells. J Comp Neurol 509:225-38
Matsuda, Takahiko; Cepko, Constance L (2007) Controlled expression of transgenes introduced by in vivo electroporation. Proc Natl Acad Sci U S A 104:1027-32
Trimarchi, Jeffrey M; Stadler, Michael B; Roska, Botond et al. (2007) Molecular heterogeneity of developing retinal ganglion and amacrine cells revealed through single cell gene expression profiling. J Comp Neurol 502:1047-65
Rowan, Sheldon; Cepko, Constance L (2005) A POU factor binding site upstream of the Chx10 homeobox gene is required for Chx10 expression in subsets of retinal progenitor cells and bipolar cells. Dev Biol 281:240-55

Showing the most recent 10 out of 34 publications