Abstract

In some urodeles, following lentectomy a new lens regenerats by the transdifferentiation of dorsal iris pigment epithelial cells (PECs). In culture, ventral PECs as well as PECs from other animals are able to transdifferentiate into a lens. The investigator proposes to use these systems to continue his studies of gene expression during lens regeneration. He has thus far identified several genes including homeobox-containing genes, fibroblast growth factor receptor-1 and Sonic hedgehog. He proposes to continue with studies on gene regulation of retinoblastoma and retinoic acid receptors. He also proposes to study induction of lens regeneration from the ventral iris in newts and from the dorsal iris of animals incapable of lens regeneration. This will be done by transfecting the gene of interest in the ventral or dorsal iris cells. These in vivo studies will be supplemented by in vitro ones to provide a more accurate picture of the mechanisms. Understanding induction of lens regeneration would be of enormous importance in pathological states where the lens must be removed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY010540-07
Application #
6518516
Study Section
Visual Sciences A Study Section (VISA)
Program Officer
Liberman, Ellen S
Project Start
1995-09-01
Project End
2004-04-30
Budget Start
2002-05-01
Budget End
2004-04-30
Support Year
7
Fiscal Year
2002
Total Cost
$167,231
Indirect Cost

  Institution

Name
University of Dayton
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
Dayton
State
OH
Country
United States
Zip Code
45469

  Publications

Keinath, Melissa C; Voss, S Randal; Tsonis, Panagiotis A et al. (2017) A linkage map for the Newt Notophthalmus viridescens: Insights in vertebrate genome and chromosome evolution. Dev Biol 426:211-218
Grajales-Esquivel, Erika; Luz-Madrigal, Agustin; Bierly, Jeffrey et al. (2017) Complement component C3aR constitutes a novel regulator for chick eye morphogenesis. Dev Biol 428:88-100
Tanaka, Hibiki Vincent; Ng, Nathaniel Chuen Yin; Yang Yu, Zhan et al. (2016) A developmentally regulated switch from stem cells to dedifferentiation for limb muscle regeneration in newts. Nat Commun 7:11069
Sousounis, Konstantinos; Qi, Feng; Yadav, Manisha C et al. (2015) A robust transcriptional program in newts undergoing multiple events of lens regeneration throughout their lifespan. Elife 4:
Keinath, Melissa C; Timoshevskiy, Vladimir A; Timoshevskaya, Nataliya Y et al. (2015) Initial characterization of the large genome of the salamander Ambystoma mexicanum using shotgun and laser capture chromosome sequencing. Sci Rep 5:16413
Shi, Jieming; Dong, Min; Li, Lei et al. (2015) mirPRo-a novel standalone program for differential expression and variation analysis of miRNAs. Sci Rep 5:14617
Kumar, Praveen Kumar Raj; Hoang, Thanh V; Robinson, Michael L et al. (2015) CADBURE: A generic tool to evaluate the performance of spliced aligners on RNA-Seq data. Sci Rep 5:13443
Hoang, Thanh V; Kumar, Praveen Kumar Raj; Sutharzan, Sreeskandarajan et al. (2014) Comparative transcriptome analysis of epithelial and fiber cells in newborn mouse lenses with RNA sequencing. Mol Vis 20:1491-517
Luz-Madrigal, Agustin; Grajales-Esquivel, Erika; McCorkle, Alexander et al. (2014) Reprogramming of the chick retinal pigmented epithelium after retinal injury. BMC Biol 12:28
Sousounis, Konstantinos; Baddour, Joelle A; Tsonis, Panagiotis A (2014) Aging and regeneration in vertebrates. Curr Top Dev Biol 108:217-46

Showing the most recent 10 out of 64 publications