Light responses of rod and cone photoreceptors are encoded by the release of glutamate-filled vesicles at photoreceptor synapses. Synaptic transmission at the first synapse in the retina thus fundamentally shapes visual perception and damage to photoreceptor synapses by protein mutation or diseases such as macular degeneration and ischemia causes vision loss. To understand the consequences of damage to these synapses and how to restore vision by therapeutic means requires a thorough understanding of their normal operation. Release from photoreceptors involves a plate-like protein structure known as the synaptic ribbon. Unlike most central nervous system (CNS) synapses that release only one or two synaptic vesicles at a time, ribbon synapses in photoreceptors and other sensory neurons are specialized for continuous release. In addition to the ribbon itself, the specialized capabilities of ribbon synapses are also determined by the use of certain proteins that differ from those at more conventional synapses. Rod and cone photoreceptors differ further from both conventional and other ribbon synapses in their use of an exocytotic Ca2+ sensor with unusual Ca2+ dependence. At most synapses, synaptic vesicle release rate rises with the 5th power of [Ca2+]i but release from photoreceptors has a weaker 1-3rd order Ca2+-dependence. The identity of the atypical Ca2+ sensor that regulates vesicle release from photoreceptors is a major unresolved question about the mechanisms of release at the first synapse in the retina. Isoforms of the protein synaptotagmin (Syt) serve as the exocytotic Ca2+ sensors in most neurons.
Our first aim i s to identify the Ca2+ sensor controlling release from photoreceptors by testing mice in which specific Syt proteins have been selectively deleted from rods or cones.
Our second aim i s to confirm that the exocytotic Ca2+ sensors in mouse rod and cone synapses retain the unusually low Ca2+ cooperativity seen in lower vertebrates.
In Aim 3, we propose to characterize how the Ca2+-dependence of release rate is shaped by different combinations of Syt, Complexin, and SNARE proteins that reproduce components of the rapid release machinery at different conventional and ribbon synapses, using unique in vitro approaches that can probe single fusion pores with sub-ms time resolution. Together, these experiments will reveal the mechanisms responsible for the atypical Ca2+-dependence of neurotransmission at the critical first synapse in vision and allow us to understand how the expression of particular proteins shapes the properties of release to meet specific signaling needs at different CNS synapses.

Public Health Relevance

Transmission of rod and cone light responses at the first synapse in retina shapes vision and damage to these synapses can cause blindness. To understand the consequences of damage and how to restore function therapeutically requires an understanding of normal synaptic operation. We aim to identify the calcium sensor protein that controls neurotransmission from rods and cones and to learn how particular synaptic proteins shape release to meet the needs of photoreceptors and other neurons in retina and brain.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY010542-24
Application #
9857594
Study Section
Neurotransporters, Receptors, and Calcium Signaling Study Section (NTRC)
Program Officer
Greenwell, Thomas
Project Start
1996-06-01
Project End
2024-01-31
Budget Start
2020-02-01
Budget End
2021-01-31
Support Year
24
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of Nebraska Medical Center
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
168559177
City
Omaha
State
NE
Country
United States
Zip Code
68198
Wen, Xiangyi; Van Hook, Matthew J; Grassmeyer, Justin J et al. (2018) Endocytosis sustains release at photoreceptor ribbon synapses by restoring fusion competence. J Gen Physiol 150:591-611
Grassmeyer, Justin J; Thoreson, Wallace B (2017) Synaptic Ribbon Active Zones in Cone Photoreceptors Operate Independently from One Another. Front Cell Neurosci 11:198
Van Hook, Matthew J; Babai, Norbert; Zurawski, Zack et al. (2017) A Presynaptic Group III mGluR Recruits G??/SNARE Interactions to Inhibit Synaptic Transmission by Cone Photoreceptors in the Vertebrate Retina. J Neurosci 37:4618-4634
Datta, Proleta; Gilliam, Jared; Thoreson, Wallace B et al. (2017) Two Pools of Vesicles Associated with Synaptic Ribbons Are Molecularly Prepared for Release. Biophys J 113:2281-2298
Thoreson, Wallace B; Van Hook, Matthew J; Parmelee, Caitlyn et al. (2016) Modeling and measurement of vesicle pools at the cone ribbon synapse: Changes in release probability are solely responsible for voltage-dependent changes in release. Synapse 70:1-14
Grishchuk, Yulia; Stember, Katherine G; Matsunaga, Aya et al. (2016) Retinal Dystrophy and Optic Nerve Pathology in the Mouse Model of Mucolipidosis IV. Am J Pathol 186:199-209
Warren, Ted J; Van Hook, Matthew J; Tranchina, Daniel et al. (2016) Kinetics of Inhibitory Feedback from Horizontal Cells to Photoreceptors: Implications for an Ephaptic Mechanism. J Neurosci 36:10075-88
Warren, Ted J; Van Hook, Matthew J; Supuran, Claudiu T et al. (2016) Sources of protons and a role for bicarbonate in inhibitory feedback from horizontal cells to cones in Ambystoma tigrinum retina. J Physiol 594:6661-6677
Cork, Karlene M; Van Hook, Matthew J; Thoreson, Wallace B (2016) Mechanisms, pools, and sites of spontaneous vesicle release at synapses of rod and cone photoreceptors. Eur J Neurosci 44:2015-27
Chen, Minghui; Van Hook, Matthew J; Thoreson, Wallace B (2015) Ca2+ Diffusion through Endoplasmic Reticulum Supports Elevated Intraterminal Ca2+ Levels Needed to Sustain Synaptic Release from Rods in Darkness. J Neurosci 35:11364-73

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