Programmed cell death is important for the development of the nervous system in general, and the visual system in particular. In the developing Drosophila compound eye, programmed cell death plays a major role at two stages: in the third instar disc, close to the morphogenetic furrow, and later, in the mid-pupa, at the end of the process of ommatidial cell recruitment, when it eliminates excess cells. A number of alleles of the roughest gene are known, and they can affect programmed cell death as well as other aspects of development. The gene roughest encodes a trans-membrane protein which can mediate homophilic adhesion when expressed on tissue culture cells, and may act as a receptor for a programmed cell death-inducing signal. Two alleles (rstCT and rst3) are weak mutations which affect the C-terminal domain of Roughest and have more specific effects on programmed cell death in the eye. The rst3 allele has been made the basis of an interaction screen to find other genes involved in retinal programmed cell death. The focus of this proposal is the study of these genes, and their developmental and molecular functions.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY011495-02
Application #
2882915
Study Section
Visual Sciences C Study Section (VISC)
Project Start
1998-03-01
Project End
2001-02-28
Budget Start
1999-03-01
Budget End
2000-02-29
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Washington University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Rudrapatna, V A; Bangi, E; Cagan, R L (2014) A Jnk-Rho-Actin remodeling positive feedback network directs Src-driven invasion. Oncogene 33:2801-6
Rudrapatna, Vivek A; Bangi, Erdem; Cagan, Ross L (2013) Caspase signalling in the absence of apoptosis drives Jnk-dependent invasion. EMBO Rep 14:172-7
Johnson, Ruth I; Bao, Sujin; Cagan, Ross L (2012) Interactions between Drosophila IgCAM adhesion receptors and cindr, the Cd2ap/Cin85 ortholog. Dev Dyn 241:1933-43
Cagan, Ross L (2011) The Drosophila nephrocyte. Curr Opin Nephrol Hypertens 20:409-15
Larson, David E; Johnson, Ruth I; Swat, Maciej et al. (2010) Computer simulation of cellular patterning within the Drosophila pupal eye. PLoS Comput Biol 6:e1000841
Bao, Sujin; Fischbach, Karl-Friedrich; Corbin, Victoria et al. (2010) Preferential adhesion maintains separation of ommatidia in the Drosophila eye. Dev Biol 344:948-56
Cordero, Julia B; Cagan, Ross L (2010) Canonical wingless signaling regulates cone cell specification in the Drosophila retina. Dev Dyn 239:875-84
Cagan, Ross (2009) Principles of Drosophila eye differentiation. Curr Top Dev Biol 89:115-35
Larson, David E; Liberman, Zoe; Cagan, Ross L (2008) Cellular behavior in the developing Drosophila pupal retina. Mech Dev 125:223-32
Seppa, Midori J; Johnson, Ruth I; Bao, Sujin et al. (2008) Polychaetoid controls patterning by modulating adhesion in the Drosophila pupal retina. Dev Biol 318:1-16

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