Past work under this grant documented the existence of a peculiar type of mammalian ganglion cell that functions as an autonomous photoreceptor. These intrinsically photosensitive retinal ganglion cells (ipRGCs) use the invertebrate-like photopigment melanopsin. They encode ambient light intensity and help to drive various reflexive responses to daylight, such resetting the circadian clock and adjusting pupil diameter. Much of the work in the next grant period is inspired by two surprising lines of pilot evidence. First, we now believe that there is at least one additional type of intrinsically photosensitive ganglion cell. Second, we have evidence that signals from ganglion-cell photoreceptors propagate not only to the non-image-forming visual networks of the brain, but also within the eye itself to other retinal neurons. Targets appear to include certain ganglion cells and dopaminergic amacrine (DA) cells. The influence of ipRGCs on DA cells is apparently reciprocated, and we will study the mutual interactions between these cells in detail. DA cells and ipRGCs are further linked by the fact that both stratify in the same OFF sublamina of the IPL, yet receive a paradoxical synaptically driven ON input of unknown origin. Here, we will work to identify a neural circuit that could account for this input.
The specific aims of the proposal are: 1) to characterize the structure and function of a new type of ganglion-cell photoreceptor; 2) to characterize the inputs to ipRGCs from dopaminergic amacrine cells and ON bipolar cells; and 3) to characterize the influences of ipRGCs on dopaminergic amacrine cells and other retinal neurons. These studies extend our understanding of the photoreceptive capacity that persists in human outer retinal degenerations such as retinitis pigmentosa. They also bear on fundamental mechanisms of circadian and adaptation modulation of retinal function. ? ? ?
| Bleckert, Adam; Zhang, Chi; Turner, Maxwell H et al. (2018) GABA release selectively regulates synapse development at distinct inputs on direction-selective retinal ganglion cells. Proc Natl Acad Sci U S A 115:E12083-E12090 |
| Stabio, Maureen E; Sabbah, Shai; Quattrochi, Lauren E et al. (2018) The M5 Cell: A Color-Opponent Intrinsically Photosensitive Retinal Ganglion Cell. Neuron 97:150-163.e4 |
| Fernandez, Diego Carlos; Fogerson, P Michelle; Lazzerini Ospri, Lorenzo et al. (2018) Light Affects Mood and Learning through Distinct Retina-Brain Pathways. Cell 175:71-84.e18 |
| Stabio, Maureen E; Sabbah, Shai; Quattrochi, Lauren E et al. (2018) The M5 Cell: A Color-Opponent Intrinsically Photosensitive Retinal Ganglion Cell. Neuron 97:251 |
| Sabbah, Shai; Berg, Daniel; Papendorp, Carin et al. (2017) A Cre Mouse Line for Probing Irradiance- and Direction-Encoding Retinal Networks. eNeuro 4: |
| Sabbah, Shai; Gemmer, John A; Bhatia-Lin, Ananya et al. (2017) A retinal code for motion along the gravitational and body axes. Nature 546:492-497 |
| Chew, Kylie S; Renna, Jordan M; McNeill, David S et al. (2017) A subset of ipRGCs regulates both maturation of the circadian clock and segregation of retinogeniculate projections in mice. Elife 6: |
| Walker, Marquis T; Rupp, Alan; Elsaesser, Rebecca et al. (2015) RdgB2 is required for dim-light input into intrinsically photosensitive retinal ganglion cells. Mol Biol Cell 26:3671-8 |
| Renna, Jordan M; Chellappa, Deepa K; Ross, Christopher L et al. (2015) Melanopsin ganglion cells extend dendrites into the outer retina during early postnatal development. Dev Neurobiol 75:935-46 |
| Lucas, Robert J; Peirson, Stuart N; Berson, David M et al. (2014) Measuring and using light in the melanopsin age. Trends Neurosci 37:1-9 |
Showing the most recent 10 out of 31 publications
