Abstract

The photoreceptor outer segment is an elaborate primary cilium. The delivery of opsin into and along the cilium is critical for photoreceptor cell function and viability. The overall goal of this study is to understand the cellular mechanisms involved in these delivery processes. A combination of cell biological methods, including imaging of protein movements in the cilium of live mouse photoreceptor cells, will be used. Studies will address questions about opsin entry into and transport along the cilium, and the membrane diffusion barrier of the photoreceptor cilium. The proposed research incorporates technical innovation, including the measurements of real-time movements of opsin along the cilium, using a ciliated epithelial cell line and mouse rod photoreceptor cells. It will test novel concepts of opsin trafficking and the organization of the photoreceptor cilium.

Public Health Relevance

The proposed research will provide a fundamental mechanistic understanding of cellular processes in the photoreceptor cilium. It will provide insight into the pathogenesis of a wide range of diseases, known as ciliopathies, which include retinal degeneration. There will also be tangible benefits to preclinical studies for retinal degeneration therapies, in that mutant phenotypes, which can be used to assess therapeutic efficacy, are likely to be identified by the research.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
2R01EY013408-07
Application #
8440269
Study Section
Special Emphasis Panel (BVS)
Program Officer
Neuhold, Lisa
Project Start
2001-09-30
Project End
2018-04-30
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
7
Fiscal Year
2013
Total Cost
$385,000
Indirect Cost
$135,000

  Institution

Name
University of California Los Angeles
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Esteve-Rudd, Julian; Hazim, Roni A; Diemer, Tanja et al. (2018) Defective phagosome motility and degradation in cell nonautonomous RPE pathogenesis of a dominant macular degeneration. Proc Natl Acad Sci U S A 115:5468-5473
Hazim, Roni A; Volland, Stefanie; Yen, Alice et al. (2018) Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide. Exp Eye Res 179:18-24
Volland, Stefanie; Williams, David S (2018) Preservation of Photoreceptor Nanostructure for Electron Tomography Using Transcardiac Perfusion Followed by High-Pressure Freezing and Freeze-Substitution. Adv Exp Med Biol 1074:603-607
Hazim, Roni A; Karumbayaram, Saravanan; Jiang, Mei et al. (2017) Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Res Ther 8:217
Williams, D S; Chadha, A; Hazim, R et al. (2017) Gene therapy approaches for prevention of retinal degeneration in Usher syndrome. Gene Ther 24:68-71
Orme, Mariam H; Liccardi, Gianmaria; Moderau, Nina et al. (2016) The unconventional myosin CRINKLED and its mammalian orthologue MYO7A regulate caspases in their signalling roles. Nat Commun 7:10972
Goldberg, Andrew F X; Moritz, Orson L; Williams, David S (2016) Molecular basis for photoreceptor outer segment architecture. Prog Retin Eye Res 55:52-81
Eblimit, Aiden; Nguyen, Thanh-Minh T; Chen, Yiyun et al. (2015) Spata7 is a retinal ciliopathy gene critical for correct RPGRIP1 localization and protein trafficking in the retina. Hum Mol Genet 24:1584-601
Jiang, Mei; Esteve-Rudd, Julian; Lopes, Vanda S et al. (2015) Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis. J Cell Biol 210:595-611
Volland, Stefanie; Hughes, Louise C; Kong, Christina et al. (2015) Three-dimensional organization of nascent rod outer segment disk membranes. Proc Natl Acad Sci U S A 112:14870-5

Showing the most recent 10 out of 27 publications