Human vision starts when photoreceptors collect and respond to light. Photoreceptors do not function in isolation though, but share close interdependence with neighboring photoreceptors, retinal pigment epithelium (RPE) cells, and choriocapillaris (CC). These close interactions underlie normal function of photoreceptors, but also the entire photoreceptor-RPE-CC complex, the primary site of most retinal dystrophies including age-related macular degeneration (AMD) and inherited diseases such as retinitis pigmentosa (RP). Techniques to assess this complex in vivo, however, are limited. New optical modalities that are rapid, specific, and non-invasive hold the promise of greatly expanding our capability to monitor more accurately and completely the photoreceptor-RPE-CC complex. This study takes advantage of unique AO and OCT instrumentation developed in my laboratory for sampling rapidly and reproducibly volume regions of the photoreceptor-RPE-CC complex at the cellular level. We will use MHz AO-OCT in conjunction with sub-cellular 3D registration and phase techniques that we have developed that are sensitive to optical length changes as small as 45 nm. We will use these techniques to investigate three specific aims: (1) determine properties of photoreceptor disc shedding, (2) evaluate disruption in the cellular surround of drusen, an early indicator of AMD, and (3) measure cell loss dynamics in RP.
This study takes advantage of unique camera technology developed in my laboratory for live imaging of the human retina, capturing exquisite details of the retina?s cellular composition in 3D. We will use this camera to investigate early changes that occur in the retina caused by age-related macular degeneration and retinitis pigmentosa. Results of this study may help improve our ability to detect earlier and monitor treatment more accurately some of the most blinding diseases in the world.
| South, Fredrick A; Kurokawa, Kazuhiro; Liu, Zhuolin et al. (2018) Combined hardware and computational optical wavefront correction. Biomed Opt Express 9:2562-2574 |
| Kurokawa, Kazuhiro; Liu, Zhuolin; Miller, Donald T (2017) Adaptive optics optical coherence tomography angiography for morphometric analysis of choriocapillaris [Invited]. Biomed Opt Express 8:1803-1822 |
| Liu, Zhuolin; Kurokawa, Kazuhiro; Zhang, Furu et al. (2017) Imaging and quantifying ganglion cells and other transparent neurons in the living human retina. Proc Natl Acad Sci U S A 114:12803-12808 |
| Marcos, Susana; Werner, John S; Burns, Stephen A et al. (2017) Vision science and adaptive optics, the state of the field. Vision Res 132:3-33 |
| Liu, Zhuolin; Kocaoglu, Omer P; Miller, Donald T (2016) 3D Imaging of Retinal Pigment Epithelial Cells in the Living Human Retina. Invest Ophthalmol Vis Sci 57:OCT533-43 |
| Kocaoglu, Omer P; Liu, Zhuolin; Zhang, Furu et al. (2016) Photoreceptor disc shedding in the living human eye. Biomed Opt Express 7:4554-4568 |
| Jonnal, Ravi S; Kocaoglu, Omer P; Zawadzki, Robert J et al. (2016) A Review of Adaptive Optics Optical Coherence Tomography: Technical Advances, Scientific Applications, and the Future. Invest Ophthalmol Vis Sci 57:OCT51-68 |
| Jonnal, Ravi S; Kocaoglu, Omer P; Zawadzki, Robert J et al. (2015) Author Response: Outer Retinal Bands. Invest Ophthalmol Vis Sci 56:2507-10 |
| Kocaoglu, Omer P; Turner, Timothy L; Liu, Zhuolin et al. (2014) Adaptive optics optical coherence tomography at 1 MHz. Biomed Opt Express 5:4186-200 |
| Jonnal, Ravi S; Kocaoglu, Omer P; Zawadzki, Robert J et al. (2014) The cellular origins of the outer retinal bands in optical coherence tomography images. Invest Ophthalmol Vis Sci 55:7904-18 |
Showing the most recent 10 out of 26 publications
